开心解郁丸对血管性抑郁大鼠单胺递质及BDNF的影响

Effects of Kaixin Jieyu Pills on monoamines and BDNF in rats with vascular depression

目的研究开心解郁丸(人参、柴胡、巴戟天,等)对血管性抑郁的治疗作用和机理。方法采用双侧颈总动脉结扎叠加慢性不可预见性温和应激和孤养方法建立血管性抑郁大鼠模型,分别连续给予开心解郁丸和氟西汀28 d。通过糖水偏好试验和旷场试验评价抑郁样行为,通过激光散斑血流检测仪视频系统测定脑血流,分别采用ELISA和Western blot法测定5-羟色胺(5-HT)、去甲肾上腺素(NA)、多巴胺(DA)等单胺递质和脑源性神经营养因子(BDNF)。结果血管性抑郁大鼠模型中,开心解郁丸可显著增加糖水消耗百分比和旷场试验中的运动距离,增加脑血流量,可不同程度地增加海马中5-HT、NA、DA的水平,促进海马组织中BDNF蛋白的表达。开心解郁丸在改善行为学、调节单胺递质和BDNF方面与氟西汀相似,而增加脑血流的功能上优于后者。结论开心解郁丸对血管性抑郁大鼠有一定的抗抑郁作用,可能与调节单胺递质及BDNF有关。

AIM To aim at investigating the function of Kaixin Jieyu Pills（Ginseng Radix et Rhizoma,Bupleuri Radix,Morindae officinalis Radix,etc.） to vascular depression and their mechanism of action.METHODS A vascular depression model of rat was established by chronic unpredictable mild stress and separation after ligation of the bilateral common carotid arteries.The rats were treated with Kaixin Jieyu Pills and fluoxetine hydrochloride（FLU） for 28 days,respectively.The sucrose preference test and open-field test were performed.Cerebral perfusion was investigated with a Perfusion Speckle Imager.The monoamines including serotonin,dopamine and noradrenaline,and brain-derived neurotrophic factor（BDNF） were determined with ELISA and Western bolt,respectively.RESULTS Kaixin Jieyu Pills significantly increased sucrose preference,moving distance and cortical blood flow.Kaixin Jieyu Pills could upregulate,to different extents,the expressions of monoamines,including serotonin,noradrenaline and dopamine,and BDNF.Kaixin Jieyu Pills had a function similar to FLU in behavior,upregulating monoamines and BDNF,but it is superior to FLU in cortical blood flow.CONCLUSION Kaixin Jieyu Pills has the advantage of reducing the depression-like behavior and improving cerebral hypoperfusion,which might be mediated by the upregulation of the serotonin,noradrenaline and BDNF.