三种生物染色剂对银边吊兰植株生长及膜脂过氧化系统的影响

Effect of Three Biological Stains on Plant Growth and Membrane Lipid Peroxidation System of Chlorophytum comosum var.variegatum

以银边吊兰为试材,研究了不同浓度(0、0.5、1.0、2.0mmol·L^(-1))的3种生物染色剂(甲基橙、甲基紫、中性红)对其植株生长及膜脂过氧化系统的影响。结果表明:经3个浓度的甲基紫及0.5mmol·L^(-1)的甲基橙和中性红处理后,银边吊兰的生物量、根系长度、叶片长度和叶片宽度均显著大于对照(CK),2.0mmol·L^(-1)处理则使以上形态指标显著小于CK,而1.0mmol·L^(-1)处理后则与CK差异不显著。处理后,叶片丙二醛、过氧化氢含量、氧自由基产生速率及过氧化物酶(POD)活性在整个试验期间均高于CK,且随着浓度的增加和时间的延长有明显的上升趋势;叶片超氧化物歧化酶(SOD)活性随着时间的延长有先上升后下降的趋势,但2.0mmol·L^(-1)处理的活性低于低浓度处理的活性,表明此时抗氧化系统已开始紊乱。综上,结合染色剂对植株生长和膜脂过氧化系统的影响来看,1.0mmol·L^(-1)的甲基橙、甲基紫和中性红比较适合银边吊兰的活体染色。

Chlorophytum cornosurn var. variegatum was used as test material, the effect of three biological stains （methyl orange, methyl violet and neutral red） of different concentration （0,0. 5,1.0,2. 0 mmol · L-1 ） on plant growth and membrane lipid peroxidation system were studied. The results showed that after treatment with the three concentrations of methyl violet and 0. 5 mmol · L-1 of methyl orange and neutral red, biomass, root length, leaf length and leaf width of Chlorophyturn comosum vat. variegatum were significantly larger than those of control （CK）,after 2. 0 mmol · L-1 treatment,the above morphological indicators were significantly less than CK,whereas after 1.0 mmol · L-1 treatment and CK were not significantly different. After treatment, MDA, H2O2 content,oxygen free radical production rate and peroxidase （POD） activity of leaves were higher than CK during the whole experiment, and with the increase of concentration and time, there was an obvious upward trend; the superoxide dismutase （SOD） increased first and then decreased with time,but the activity of 2. 0 mmol · L- 1 treatment was lower than that of low concentration, which indicated that the antioxidant system was beginning to disorder. Thus, combined with the effect of dying agent on plant growth and membrane lipid peroxidation system, 1.0 mmol·L-1 methyl orange,methyl violet and neutral red were more suitable for Chlorophyturn comosum var. variegatum intravital staining.