笃斯越桔高效再生体系的建立

Efficient Regeneration System for Vaccinium uliginosum L.

以笃斯越桔带叶嫩茎为外植体,研究植物生长调节剂对其叶片、茎段等不定芽再生及组培苗瓶内生根壮苗的影响;探索不同移栽技术对组培苗移栽成活率的影响。结果表明:玉米素(ZT)能够促进叶片、茎段等外植体高效分化出不定芽,联合使用吲哚丁酸(IBA)能够降低增殖系数,使不定芽叶片更绿、茎较粗壮,MWPM+1.0mg·L^(-1) ZT+0.1mg·L^(-1) IBA为最佳不定芽再生培养基;且低浓度的IBA能有效促进组培苗生根,矮壮素(CCC)具有壮苗作用,MWPM+0.5mg·L^(-1) IBA+5.0mg·L^(-1) CCC为最佳壮苗生根培养基;该研究提出的育苗袋辅助移栽技术,使组培苗移栽成活率达到100%,建立了笃斯越桔高效再生体系。

The tender stem explants with leaves from Vaccinium uliginosum L.were cultured on medium supplemented with different type,concentration and combination of plant growth regulators to study their effects on adventitious buds regeneration and rooting and shoot growth,respectively.And the effects of different transplanting techniques on the survival rate of plantlets were studied as well.The results indicated that ZT could promote the differentiation of adventitious buds from explants such as leaves and stem segments.The multiplication coefficient was reduced and the adventitious buds were more green and stout when explants were cultured on medium with IBA.The best medium for adventitious buds regeneration was MWPM＋1.0 mg·L-1 ZT＋0.1 mg·L-1 IBA.Low concentration of IBA could effectively promote the rooting rate of shoots and chlormequat could make the seedlings more strong so that the optimum medium for rooting and strong seedling was MWPM＋0.5 mg·L-1 IBA＋5.0 mg·L-1 CCC.In the process of transplanting the survival rate reached 100% when the transplanting technique assisted with the nursery bag was used.The results showed that an efficient regeneration system was established for Vaccinium uliginosum L.in this study.