广藿香不同药用部位挥发油物质基础差异与抗白色念珠菌的相关性研究

Composition Differences of Volatile Oil from Different Parts of Pogostemon cablin and Its Correlation with Anti-fungus Activity Against Candia Albicans

为了阐明广藿香叶挥发油、地上部分挥发油及带根全株挥发油的物质基础差异与其抗白色念珠菌活性差异的相关性。采用GC-MS法建立指纹图谱,对三组共有成分进行检识和聚类分析,比较指纹图谱相似度,分析物质基础差异;用倍比稀释法测定其抗白色念珠菌活性,用灰色关联分析法研究其谱效关系。结果表明,除叶组外,地上组与全株组聚类分析结果一致,且指纹图谱相似度较好;全株组除广藿香酮相对百分含量增幅较大以外,其它成分种类及含量与地上组基本一致,而叶组几乎不含广藿香酮;抗白色念珠菌活性:全株组>地上组>叶组;广藿香酮与抗白念珠菌活性相关度最高。由此可见,如果将带根全株取代地上部分作为广藿香的药用部位,不仅未改变原挥发油物质群,而且提高了广藿香酮含量,增加了抗白念珠菌的效果。

To elucidate the differences of material base of volatile oils from the leaves, aerial parts and whole part of Pog- ostemonis herba and its correlation with anti-Candida albicans activity. The fingerprints of volatile oils were established by GC-MS. The common peaks of the three oils were relatively recognized and cluster analyzed by SPSS software to compare the fingerprint similarity and analyze of material differences. The anti-C, albicans activity was detected by double dilution method and the correlation with fingerprint was identified by grey relational analysis. The cluster analysis of the other two oils was found to be divided into the same categories and the similarities were perfectly correlated, except for the oil of leaves. Comparing with the aerial part, the whole plants had almost no change in the types and contents of the components except the drastically enhancing of patehoulenone. The oil of leaves contained no patchoulenone. The efficacy order of anti-C, albicanswas as follows ： the oil of whole part 〉 the oil of aerial parts 〉 the oil of leaves, and patchoulenone had the highest correlation with anti-C, albicans activity. In conclusion, the use of the whole plant instead of the aerial parts of P. cablin did not change the original components of the volatile oil, but increased the patchoulenone content. Furthermore, it also improved the effect of anti-C, albicans effect.