丹酚酸A的分离纯化工艺及其抗氧化活性的研究

Separation,Purification and Antioxidant Activity of Salvianolic Acid A from Salvia miltiorrhiza var. alba

研究优化聚酰胺树脂分离纯化白花丹参丹酚酸A的最佳工艺,并测试其提取物的抗氧化活性。以吸附量和解吸量为指标,利用静态吸附和动态吸附的方法,确定丹酚酸A的最佳分离纯化条件。结果表明聚酰胺树脂的最佳纯化工艺条件为:上样液丹酚酸A质量浓度为11 g/L,上样液体积流量为1.0 m L/min,上样量为150m L,洗脱溶剂为50%乙醇,洗脱体积流量为1.0 m L/min,洗脱体积为10 BV,纯化后丹酚酸A量可达40.36%。通过清除DPPH自由基和还原能力测定初步评判该工艺下的提取物的抗氧化活性,结果表明,该提取物具有良好的抗氧化活性。

To optimize the separation and purification technology of salvianolic acid A from Salvia miltiorrhiza var. alba with polymide resin. The antioxidant activity of the purified salvianolic acid A were studied in vitro. With adsorption capacity and desorption rate as index, static and dynamic adsorption-desorption were used to determine the optimal separation and purification conditions of salvianolic acid A. The optimal conditions of polymide resin were as follows ： sample injection concentration was 11 g/L,sample flow rate was 1.0 mL/min,quantity of adsorption was 150 mL, desorption rate was 1.0 mL/min, and elution volume was 10 BV of 50% ethanol. After the purification, the purity of obtained salvianolie acid A was 40.36%. Moreover,the antioxidant activity of the extract were evaluated by DPPH scavenging assay and reducing power. The results showed that the obtained salvianolic acid A had good antioxidant activity.