乳酸菌发酵黄芪多糖制剂在肉鸡生产上的应用

Studies on Application of Astragalus Polysacharin Fermented by Lactic Acid Bacteria In Broilers

本试验旨在研究一种乳酸菌发酵的黄芪多糖(APS)制剂及其对肉鸡生长性能、血清免疫球蛋白Ig G、Ig A含量及肠道菌群的影响。首先提取APS,测定乳酸菌对其发酵的可能性,确定最适宜的添加量;其次选择500只1日龄体重相近的健康AA^+肉仔鸡,随机分为5组,每组100只。Ⅰ组为对照组,饲喂基础日粮,试验Ⅱ、Ⅲ、Ⅳ、Ⅴ组分别在基础日粮中添加20%APS 0.1ɡ/kg和0.1、0.2、0.3 m L/kg的乳酸菌发酵制剂。试验期为6周。结果表明:乳酸菌在添加20%APS的液体培养基中生长最优;与对照组相比,试验Ⅲ、Ⅳ、Ⅴ组肉鸡的生长性能显著提高(P<0.05),其中以Ⅳ组效果最好;与对照组相比,试验Ⅲ、Ⅳ、Ⅴ组肉鸡血清Ig G、Ig A含量显著提高(P<0.05),试验组肉鸡肠道微生物体系均有改善,盲肠大肠杆菌数量显著降低(P<0.05),盲肠乳酸菌数量显著升高(P<0.05)。综合考虑,肉鸡日粮中乳酸菌发酵APS制剂的最佳添加量为0.2 m L/kg。

This study was aimed to prepare of a kind ofAstragalus Polysacharin（APS） which fermented by lactic acid and research its influence on the growth of broiler, serum immunoglobulin levels and intestinal flora. Firstly, the preparation of APS, as well as the feasibility of fermenting Astragalus using lactic acid bacteria was determined and the best optimum adding amount. Secondly, 500 1-day-old AA^＋ broilers were randomly divided into 5groups. Chickens in the control group were fed basal diet, while chickens in the test groups, based upon the basal diet, were added with 0.1 g/kg of the 20% APS and 0.1, 0.2, 0.3 mL/kg of preparation which fermented by lactic acid. And the experiment lasted for 6 weeks. The results showed that 20% APS has better growth-promoting effects to the lactic acid. During the whole test period, compared with control group, groups Ⅲ, Ⅳ and Ⅴ was significantly improved the growth performance of broilers （P〈0.05）, as well as group IV was the best; Compared with control group, groups Ⅱ, Ⅲ, Ⅳ and Ⅴ was significantly improved the content of IgG and IgA （P〈0.05）, groups Ⅱ, Ⅲ, Ⅳ and Ⅴ could make the intestinal micro environment better, reduce the number of E.coli in cecum, and increase the number of Lactobacillus in broilers and the effect was significant（P〈0.05）. In conclusion, based upon the basal diet, the best addition amount of the preparation which fermented by lactic acid was 0.2mL/kg.