沉默/增强Stat1对胰腺癌细胞增殖和迁移的影响

Effect of silenced/enhanced Stat1 gene on proliferation and invasion in pancreatic cancer cells

目的研究沉默/增强Stat1对胰腺癌细胞增殖和迁移能力的影响。方法将胰腺癌细胞(Bx PC-3)分为:对照(control)组、Stat1组和si Stat1组,分别转染Stat1质粒和特异性干扰Stat1表达的siRNA;Western blot法检测Bx PC-3细胞中Stat1、VEGF、MMP-2和MMP-9蛋白的表达;MTT法绘制细胞增殖曲线;Transwell法检测Bx PC-3细胞侵袭能力。结果沉默Stat1表达后,Bx PC-3细胞增殖及迁移能力明显增加,且VEGF、MMP-2和MMP-9表达明显增加。而增强Stat1表达后,Bx PC-3细胞增殖及迁移能力明显降低(P<0.05)。结论 Stat1可以抑制Bx PC-3细胞增殖及迁移能力。且VEGF、MMP-2和MMP-9可能在Stat1抑制肿瘤过程中起一定作用。

Objective To investigate the proliferation and invasion of human pancreatic cancer cells after Statl gene silenced or enhanced and to explore the possible mechanisms. Methods BxPC-3 cells were divided to three groups： Statl group transfected with specific Statl plasmid, Si Statl group transfected with specific Statl small interfering RNA （ siRNA）, and control group. Western blot was used to detect the expression of Statl, VEGF, MMP-2 and MMP-9 in BxPC-3 cells. MTT method was used to detect the proliferation of BxPC-3 cells. Transwell was used to detect the invasion of BxPC-3 cells. Results The proliferation and invasion were significantly enhanced in pancreatic cancer cells after Statl gene silenced, and the expression of VEGF, MMP-2 and MMP-9 was increased. The proliferation and invasion were significantly decreased in pancreatic cancer cells after Statl gene enhanced. Conclusions Statl inhibits the proliferation and invasion of pancreatic cancer cells. VEGF, MMP-2 and MMP-9 possibly play a role in this process.