摘要
目的:初步建立健脾益肾方的质量控制方法。方法:采用薄层色谱法鉴别黄芪、白术、大黄、肉苁蓉,并对毛蕊异黄酮葡萄糖苷进行含量测定研究。Agilent Extend-C18;流动相:乙腈:0.05%磷酸(13:87):检测波长:260nm,柱温:25℃,流速0.8mL/min。结果:薄层色谱斑点清晰,阴性无干扰;毛蕊异黄酮葡萄糖苷在浓度5.03~40.24μg/mL范围内线性关系良好,r=0.9999,平均加样回收率为97.10%,RSD为1.15%(n=9)。结论:该方法简便、准确、可靠,可用于健脾益肾方的质量控制。
Objective: To establish the quality control method for Jianpi Yishen formula. Methods: Identified Astragalus, Atractylodes Rhizome, Rhubarb and Cistanche by layer chromatography, and determined the content of calycosin-7-glucoside. Agilent Extend-C 18; mobile phase: acetonitrile: 0.05% phosphoric acid (13:87); detection wavelength: 260nm, column temperature: 25℃, flow rate 0.8mL/min. Results: The TLC spots were clear, negative control without interference; it showed that the linear range was good at the concentration of 5.03-40.241ag/mL, r=0.9999, the average re- covery rate was 97.10%, RSD was 1.15% (n=9). Conclusion: This method is simple, accurate and reliable, which can be used in the quality control of Jianpi Yishen formula.
出处
《按摩与康复医学》
2017年第11期75-78,共4页
Chinese Manipulation and Rehabilitation Medicine
基金
2015年深圳市科创委项目
编号:JCYJ20150401163247213
关键词
健脾益肾方
薄层色谱法
高效液相色谱法
毛蕊异黄酮葡萄糖苷
质量控制
Jianpi Yishen formula
thin layer chromatography (TLC)
high performance liquid chromatography (HPLC)
calycosin-7-glucoside
quality control