基于RRS的人血清白蛋白快速检测系统设计

Rapid Detection System for HSA Based on RRS

在临床中人血清白蛋白浓度的快速检测对多种重大疾病的诊断具有重要意义,而现有的医疗检测设备普遍存在体积大、测试周期长、操作复杂等缺点,设计研究一种快速的、高精度的便携式人血清白蛋白浓度检测系统具有非常广阔的应用前景。设计了基于共振瑞利散射光谱分析的人血清白蛋白浓度检测系统。由于人血清白蛋白与四氨基酞菁铜反应产生的溶液在475.0nm处具有共振瑞利散射增强的效果,从而构建了共振瑞利散射强度与人血清白蛋白浓度的函数。实验采用紫外半导体激光器与475.0nm窄带滤光片联用,配合高增益光电探测器采集散射光信号。采用不同浓度的四氨基酞菁铜溶液分别对不同浓度的人血清白蛋白进行检测,实验结果显示,响应电压会随人血清白蛋白浓度的提高而明显增大,而对四氨基酞菁铜的浓度变化并不敏感;散射光产生的响应电压与人血清白蛋白浓度在0.1~1.0μg·mL^(-1)范围内基本满足线性变化,满足快速检测的设计要求。

In the clinic, for the diagnosis of a variety of major diseases, it has great significance in terms of quick detection of concentration of human serum albumin （HSA）. However, there are bulky, long test cycle, complex operations and other shortcomings for common medical detection equipment. It will have a very broad application prospects as for the design of a fast, high-precision portable detection system for HSA concentration. HSA concentration detection system was designed based on Resonance Rayleigh Scattering（RRS） spectroscopy. The mixed solution was generated with HSA and Tetraamino-phthalocyanine copper （CuTAPc） reaction, and it had a RRS enhancement effect at 475.0 nm, whereby the function of RRS intensity and HSA concentration was constructed. In the experiment, ultraviolet semiconductor lasers and 475.0 nm narrowband filter was used, the scattered light signal was collected with high gain photo-detector. Different concentrations of HSA were respectively detected with different concentrations of CuTAPc. Experimental results show that response voltage will increase significantly with the increasing of concentrations of HSA，but it is not sensitive to concentration changes for CuTAPc; It basically meet the linear change by the response voltage from scattered light and the concentration of HSA in the range from 0.1 to 1.0 μg·mL-1, and it meets the design requirements of rapid detection.