基于功能化的金纳米的共振瑞利散射方法手性识别肉碱对映体（英文）

A Selective Resonance Rayleigh Scattering Method for Chiral Recognition of Carnitine Enantiomers Based on Cu^(2+) Functionalized Gold Nanoparticles

一种不经分离而同时测定手性对映体的简便方案是非常有趣和有用的。提出一种基于共振瑞利散射(RRS)光谱技术手性识别新方法,利用功能化的金纳米粒子(Au NPs)同时检测肉碱对映体。AuNPs的RRS强度很弱,但当Cu^(2+)存在时,RRS强度显著增加。更有趣的是,肉碱对映体均可以降低Cu^(2+)-Au NPs体系的RRS强度,但D-肉碱使RRS降低更多。在最优实验条件下,均有良好的线性关系并有很好的相关系数以及较低的检出限。由此,这种方法可以计算出肉碱对映体的对映体比率和对映体分数。并应用于胶囊样品中肉碱对映体混合物手性识别的研究。该方法不需要复杂的手性修饰处理,并具有简捷低消耗、灵敏度高、选择性好等优点。

A simple protocol to simultaneously determinate chiral enantiomers without separation is of great significance. Herein, we reported a method for chiral recognition was proposed. Cu2＋ functionalized gold nanoparticles （Au NPs） was used as a sensor for carnitine enantiomers based on the resonance Rayleigh Scattering （RRS） spectral technique. The RRS intensity of Au NPs is very weak, while RRS highly increased in the presence of Cu2＋. More interestingly, carnitine enantiomers could quench the RRS intensities of Cu2＋-Au NPs system, and they had different quenching degree. In the optimum experimental conditions, good linear relation, high correlation coefficient and low detection limit could be obtained. In addition, this proposed method could obtain enantiomer ratios and enantiomer fraction of carnitine enantiomers. And the applicability of the chiral recognition of carnitine enantiomers mixtures in capsule samples had been demonstrated. Particularly, this method does not require complicated chiral modification and excels through its low-cost, sensitivity, enantioselectivity, simplicity and good availability of materials.