HPLC法测定牛蒡中牛蒡子苷、牛蒡子苷元、绿原酸的含量

Simultaneous Determinate the Content of Arctiin,Arctigenin and Chlorogenic acid in Arctium lappa L. by HPLC

目的:建立高效液相色谱法(HPLC)同时测定牛蒡中牛蒡子苷、牛蒡子苷元、绿原酸含量的方法,比较3种成分在不同部位以及牛蒡子不同炒制时间的含量。方法:采用Welchrom C_(18)柱(5μm,4.6mm×250mm);流动相为甲醇-0.1%磷酸水梯度洗脱;流速为1.0m L/min;检测波长为280nm;柱温为30℃。结果:3种成分的进样量分别在4.2844~51.4128μg,0.3254~3.9048μg,0.3072~3.6864μg范围内呈良好的线性关系,平均加样回收率分别为99.64%,100.81%,97.92%,RSD值均小于1.5%;牛蒡叶和牛蒡根中几乎没有牛蒡子苷和牛蒡子苷元;牛蒡子所有炒制品与生品比较,三者的总含量均下降,炒制时间从10~40min时,牛蒡子苷、绿原酸的含量下降,牛蒡子苷元的含量增加。结论:该方法简便、准确,精密度、重复性良好,可为牛蒡子质量控制提供依据,不同部位、炒制时间结果可以为牛蒡综合开发提供依据。

Objective To establish a HPLC method for the content determination of Arctiin , Arctigenin and Ch]orogenie acid in Arctium lappa L. and to make a comparison of content of this three components among different parts as well different time that Arcti- iFruetus fried. Methods The separation was carried on Welehrom C ls （51.Lm , 4. 6ram X 250ram） column with mobile phase consisted of acetonitriie -0. 1% phosphoric （gradient elution） at the flow rate of 1.0mL ~ rain- i The detection wavelength was set at 280nm. The column temperature was 30℃. Results The linear range were 4. 2844 - 51. 41281.Lg for Arctiin, 0. 3254 -3.9048μg for Arctigenin and 0. 3072-3. 6864μg for Chlorogenie acid. Average recovery rates were 99. 64%, 100. 81% , 97. 92% , with RSD less than 1.5%. There was little Arctiin and Arctigenin both in Arctium lappa leaves and Arctium lappa root. Compared with ArctiiFmctus that WaSh＇ t fried, total content of three components in fried ArctiiFmctus decreased. Content of Arctiin and Chlorogenic acid decreased while Arctigenin increased when frying time changed from 10 minutes to 40 minutes. Conclusion The HPLC method is accurate, relia- ble and reproducible to analyze three components in Arctium ]appa L , which can provide the scientific basis for formulating the quality standard of ArctiiFmetus. Results of different parts as well different time that ArctiiFmetus fried can provide the scientific basis for comprehensive development of Arctium lappa L.