RTCA实时监控SAHA对乳腺癌MCF-7细胞生长状态的影响

RTCA Monitors the Inhibitory Effect on Proliferation of Breast Cancer MCF-7 Cells with SAHA Treatment

目的:应用实时无标记动态细胞分析(RTCA)技术研究SAHA抑制乳腺癌MCF-7细胞生长的情况。方法:采用RTCA系统动态监测不同剂量(0、0.5、1、2、5、10、20、50μmol/L)SAHA对乳腺癌MCF-7细胞生长的影响。结果:5、10μmol/L的SAHA孵育48 h后可显著抑制细胞的生长,为SAHA抑制MCF-7细胞生长的最佳实验剂量和作用时长;而相关剂量的DMSO无抑制作用。结论:RTCA系统可准确地监测SAHA抑制乳腺癌MCF-7细胞生长的情况。

Objective： To observe the inhibitory effect on proliferation of breast cancer MCF-7 cells with SAHA treatment by real-time cell analysis （RTCA） system. Method： RTCA was used to monitor the proliferation of breast cancer MCF-7 cells treated with different doses of SAHA （0, 0.5, 1, 2, 5, 10, 20, 50μmol/L） . Results： With 5, 10 μmol/L dose for 48 h treatment, SAHA significantly inhibited the proliferation of MCF-7 cells, Conclusion： RTCA system accurately monitors the inhibitory treatment.