摘要
目的:应用实时无标记动态细胞分析(RTCA)技术研究SAHA抑制乳腺癌MCF-7细胞生长的情况。方法:采用RTCA系统动态监测不同剂量(0、0.5、1、2、5、10、20、50μmol/L)SAHA对乳腺癌MCF-7细胞生长的影响。结果:5、10μmol/L的SAHA孵育48 h后可显著抑制细胞的生长,为SAHA抑制MCF-7细胞生长的最佳实验剂量和作用时长;而相关剂量的DMSO无抑制作用。结论:RTCA系统可准确地监测SAHA抑制乳腺癌MCF-7细胞生长的情况。
Objective: To observe the inhibitory effect on proliferation of breast cancer MCF-7 cells with SAHA treatment by real-time cell analysis (RTCA) system. Method: RTCA was used to monitor the proliferation of breast cancer MCF-7 cells treated with different doses of SAHA (0, 0.5, 1, 2, 5, 10, 20, 50μmol/L) . Results: With 5, 10 μmol/L dose for 48 h treatment, SAHA significantly inhibited the proliferation of MCF-7 cells, Conclusion: RTCA system accurately monitors the inhibitory treatment.
出处
《沈阳医学院学报》
2017年第3期193-195,共3页
Journal of Shenyang Medical College
基金
国家自然科学基金项目(No.81172509)
关键词
乳腺癌
MCF-7
SAHA
RTCA
breast cancer
MCF-7
SAHA
RTCA while no dose-dependent effect in the same doses for the DMSO. effect on proliferation of breast cancer MCF-7 cells with SAHA