一种新型大鼠动脉移植模型的建立

A new model of rat artery transplantation

目的移植物血管病(allograft vasculopathy,AV)限制着移植器官功能及远期存活,是器官移植领域最迫切需要解决的世界性难题。传统的血管缝合方式需要优秀的显微外科技术,开展困难。探索出更为简便、可行的动脉移植方法,可为临床研究AV提供思路。方法采用静脉留置针管自制血管套管,取供体大鼠胸主动脉牵拉入套管并两端翻转、结扎固定为移植体,将该移植体植入受体大鼠腹主动脉并于造模后1周、4周、8周取移植段动脉行HE染色,观测移植血管形态、测量内膜厚度。结果 40只受体大鼠中有38只健康存活达所需检测时间点,下肢及尾巴活动自如、大小便正常,且HE染色可见移植段血管内膜表现为与AV一致的同心圆样增厚。结论此种大鼠动脉移植方法简便可行,较传统血管缝合方法可明显缩短腹主动脉阻断时间、减轻受体大鼠的手术创伤、造模成功率高,可复制性强。

Objective Allograft Vasculopathy（AV）, which limits the function and long-term survival of transplanted organs, it is the most urgent problem need to be solved in the field of organ transplantation.Traditional vascular anastomosis requires excellent microsurgical techniques, it is difficult to carry out.To explore a simple and feasible AV model can provide ideas for clinical research.Methods We make vessel cannulas by using vein detained needles, pulling the thoracic aorta of donor rat in vessel cannula, both ends turned over and fixed,and then transplant it in recipient rat abdominal aorta.1, 4 and 8 weeks after transplantation, we take out of the transplanted arteries, HE stain, to observe the histomorphology and measurement the vascular intimal thickness.Results 38 of the 40 recipient rats healthy survival to the detection time points, the legs and tail can move freely, defecating and urinating normally.It is found that transplanted arteries concentric circles thickening, its histomorphology just like the AV by using HE staining.Conclutions The method of rats artery transplantation is simple and feasible, significantly shorten the time of abdominal aortic blocking, reduce surgical trauma, can improve the success rate and the reproducibility of operation.