光固化酸蚀剂对脱敏剂封闭牙本质小管作用的影响研究

Effect of photocurable etching agent on the desensitizer on dentinal tubules

目的全酸蚀与自酸蚀粘接系统处理下脱敏剂对牙本质小管封闭效果的研究。方法搜集新鲜拔除的无龋坏牙齿制作成牙本质模型,共分四组:A组:氢氧化钙Lonosit Baseline组;B组:Gluma脱敏剂组;C组:奥威尔脱敏剂组;D组:空白对照组。先按分组不同,使用相应的脱敏剂处理牙本质模型表面,每组再分别用Single Bond 2和SE Bond处理牙本质表面。扫描电镜下观察全酸蚀与自酸蚀粘接系统处理下脱敏剂对牙本质小管的封闭效果。结果扫描电镜下见,使用自酸蚀粘接剂或全酸蚀粘接剂处理下,同种脱敏剂对牙本质小管的封闭效果基本相同。氢氧化钙组样本的牙本质表面粗糙,仅部分小管内有沉积物封闭,管口大部分外露。Gluma组样本牙本质表面平坦,少数牙本质小管管口外露,沉积物几乎全部堵塞牙本质小管,结合较紧密。奥威尔组—牙本质小管大部分都有沉积物,其小管管径明显缩小。对照组样本牙本质表面可见牙本质小管开口完全敞开,管口无阻塞或覆盖物。纵截面小管内无沉积物,可见网状交织的管间牙本质胶原纤维清晰,小管管径增大明显。结论使用自酸蚀粘接剂或全酸蚀粘接剂处理下,同种脱敏剂对牙本质小管的封闭效果基本相同,都能较好地封闭牙本质小管。其中,奥威尔脱敏剂对牙本质小管的封闭效果最好。

Objective To study the effect of desensitizer on dentinal tubule closure under total etching and self-etching bonding system. Methods Freshly removed teeth without caries were collected to make dentin model and divided into four groups including group A（Calcium hydroxide Lonosit Baseline group）, group B（Gluma desensitization group）, group C（Oravive desensitization group） and group D（control group）. The surface of dentin model was treated with the corresponding desensitizer according to the different grouping. And the dentin surface was treated with Single Bond 2 and SE Bond, respectively. The effect Of desensitizer on dentinal tubules under total etching and self-etching bonding system was observed under scanning electron microscop＇y（TEM）. Results Under the scanning electron microscope, the effect of the same desensitizer on the dentinal tubules was similar under total etching and self-etching bonding system. The dentin surface of samples in Calcium hydroxide group was rough, with sediment in only part of the tubules and expo- sure of most of the mouth. The dentin surface in Gluma group was fiat, with a small number of dentin tubules mouth exposed and almost all of dentin tubules blocked with the deposits, and the bonding was close. Most of the dentinal tubules in Oravive group had sediment,and the small tube diameter significantly reduced. In the control group, the dentin tubules were open and the mouth was free of obstruction or covering on the surface. There was no sediment in the longitudinal section of the tube, and the interstitial collagen fibers were clear and the diameter of the tubules in- creased obviously. Conclusion The effect of the same desensitizer on the dentin tubules is similar under total etching and self-etching bonding system, and the dentinal tubules can be well sealed. Among them, the effect of Oravive de- sensitizer on dentinal tubules is the best.