uH2B在原发性结肠腺癌中的表达及其与临床病理参数的关系

Expression of uH2B in primary adenocarcinoma of the colon and its relationship with clinicopathological parameters

目的探讨原发性结肠腺癌组织中组蛋白H2B单素泛化(uH2B)的表达水平及与患者临床病理参数的关系。方法选取2013年1月—2016年1月本院胃肠外科手术切除的83例结肠腺癌组织标本、癌旁组织,既往收集的20例正常结肠组织,采用免疫组化染色观察各组组织标本中uH2B蛋白的表达程度,并分析与原发性结肠腺癌患者临床病理参数的关系。结果结肠癌组织标本中uH2B蛋白的阳性表达率46.99%,低于癌旁组织的83.13%、正常结肠黏膜组织的90.00%,差异均具有统计学意义(P<0.05);癌旁组织和正常结肠黏膜组织中uH2B蛋白的阳性表达率差异无统计学意义(P>0.05);结肠癌组织标本中uH2B蛋白的阳性表达率与肿瘤的TNM分期、分化程度、淋巴结转移相关(P<0.05),与患者的年龄、性别、肿瘤浸润深度、肿瘤最大径的关系不显著(P>0.05)。结论结肠癌组织标本中uH2B蛋白表达率降低,并且与患者的肿瘤的TNM分期、分化程度、淋巴结转移具有相关性。

Objective To study of primary colorectal adenocarcinoma histone H2B single prime generalization （uH2B） and its relationship with clinicopathological parameters. Methods Colonic cancer tissue samples and para-carcinoma tissue of gastrointestinal surgery resection of 83 cases were selected from January 2013 to January 2016 in our hospital, and normal colon tissue of 20 cases was collected, and uH2B protein expression level in tissue samples was observed using immunohistochemical staining, and its relationship with clinical pathological parameters of patients with primary colonic carcinoma was analyzed. Results The positive expression rate of uH2B protein in colon cancer tissues was 46.99% , which was lower than 83.13% of adjacent tissues and 90.00% in normal colon mucosa, the differences were statistically significant （P 〈 0.05 ） ; But there was no significant difference in the positive expression of uH2B protein in adjacent tissues and normal colonic mucosa （P 〉0.05） ; The positive expression rate of tumor uH2B protein in colon cancer tissues in the TNM stage was correlation with degree of differentiation and lymph node metastasis （P 〈 0.05 ）, and it had no significant relationship with the patient＇s age, gender, tumor invasion depth, tumor size （P 〉 0.05）. Conclusion The decreased expression rate of uH2B protein in colon carcinoma tissue is correlated with the TNM stage, differentiation degree and lymph node metastasis of the patients.