基于可控定向PVDF电纺纤维构建细胞相容性的三维微环境（英文）

Tuneable Three-dimensional Cytocompatible Micro-environments Designed by Controlled Alignment of PVDF Electrospun Fibres

三维电纺纤维在生物医学领域,如生物传感、药物控制释放与组织工程等方面具有良好的应用前景.然而,现有的电纺技术在制备结构、孔隙率与形貌均可调节的三维定向电纺纤维方面还存在一定不足.因此亟需开发一种新型的电纺丝工艺以制备三维定向电纺纤维.本文通过改进传统的电纺丝工艺,开发了一种简单高效制备三维定向聚偏氟乙烯(PVDF)的电纺丝制备技术.所制备的三维定向纤维的形貌、直径及纤维密度均可控.体外细胞实验结果表明,该类三维定向纤维具有良好的生物相容性,能够促进细胞活性,诱导细胞沿着纤维的方向生长.此外,研究结果还表明,将该三维定向纤维作为细胞培养支架时,细胞的增殖高于利用传统的二维纤维膜.该制备技术将极大地拓宽三维定向纤维在三维细胞培养、组织工程及疾病诊断等生物医学领域的应用.

Three dimensional（3D） aligned electrospun fibres have a promising potential application in biomedical areas, such as biosensors, controlled drug release and tissue engineering. However, the fabrication of these fibres with tuneable microarchitectures, porosity and overall morphology still has challenges due to eleetrospinning process.Thus, it is highly desired to develop novel, cost-effective and easily scalable fabrication methods for the 3D aligned electrospun fibres. Herein, we developed a facile yet effective method for the preparation of 3D poly （ vinylidene fluoride） （PVDF） aligned fibres（3D AFs） via an improved electrospinning technique. The obtained 3D AFs showed controllable morphology, diameter and fiber density. Furthermore, the obtained 3D AFs showed excellent in vitro bioeompatibility. Moreover, the as-prepared 3D AFs enhanced cellular activities and induced directional cell growth along the direction of nanofibre orientation, thereby providing an excellent cue for the anchorage and migration of human mesenchymal stem cells （hMSCs）. More importantly, cell proliferation in the 3D AFs was found to be significantly higher than that on the nanofibre mats （NFMs）. Combined with controllable morphology and structure, we anticipate that this finding greatly enhances the potential applications of these 3D AFs for therapeutically relevant 3D cell cultures, tissue engineering, diagnostics and other biomedical applications.