三七内生真菌Talaromyces Purpureogenus中2个Drimane倍半萜绝对构型的鉴定

Absolute Configuration Determination of Two Drimane Sesquiterpenoids from the Endophytic Fungi Talaromyces Purpureogenus of Panax notoginseng

采用经典柱色谱法从三七内生真菌Talaromyces purpureogenus中分离得到2个化合物(1和2),经核磁共振波谱和质谱确认其结构均为Drimane倍半萜.通过量子化学计算比较电子圆二色谱(ECD)、旋光(OR)与实验值的方法鉴定了化合物1和2的绝对构型.生物活性测试结果显示,化合物1对大肠杆菌(E.coli)具有一定的抗菌活性(MIC=25μmol/L).

Two drimane sesquiterpenoids （1, 2 ） were isolated from cultures of the fungus Talaromyces purpureogenus. The planar structures were well established by NMR and mass spectrometry, and the absolute configurations（ AC ） were determined by comparing experimental electronic circular diehroism （ECD） and optical rotation（OR） of compounds 1 and 2 with the calculated ECD and OR data using quantum methods. Compounds 1 and 2 were evaluated for antibacterial activities against four spices of pathogenic bacteria, and the results showed that compound 1 had weak antibacterial activities against Escherichia coli（ MIC = 25 μmol/L）.