长链非编码RNASH3PXD2A-AS1在胃癌术后肝转移诊断中的预测价值

Predictive value of long chain noncoding RNA SH3PXD2A - AS1 in the diagnosis of postoperative liver metastasis of gastric cancer

目的探讨长链非编码RNASH3PXD2A-AS1在胃癌术后肝转移诊断中预测价值。方法收集37例胃癌及其配对正常胃黏膜组织，采用荧光实时定量聚合酶链反应（PCR）方法检测组织中SH3PXD2A—AS1表达水平，分析其表达与患者性别、年龄、肿瘤部位、肿瘤直径、组织分化程度、肿瘤浸润深度、淋巴结转移、肝转移数目及肝转移发生时间等临床病理因素的关系。并作统计学分析处理。结果在37例胃癌组织中SH3PXD2A—AS1阳性率为89．19％（34／37），而正常胃组织SH3PXD2A—ASI阳性率为5．41％（2／37）。两者差异有统计学意义（x^2=55．392，P=0．000）。SH3PXD2A—AS1表达量与患者性别（x^2=1．718，P=0．190）、年龄（x^2=0．560，P=0．756）、肿瘤位置（x^2=2．839，P=0．242）、组织分化程度（x^2=0．924，P=0．336）均无明显相关，但与原发灶直径（x^2=4．788，P=0．029）、肿瘤浸润深度（x^2=8．815，P=0．012）、淋巴结转移（x^2=6．801，P=0．009）、肝转移数目（x^2=10．157，P=0．001）、肝转移发生时间（x^2=6．903，P=0．032）均显著相关。结论SH3PXD2A-AS1在胃癌术后肝转移诊断中具有预测价值。

Objective To investigate the predictive value of long chain non coding RNA SH3PXD2A - AS1 in the diagnosis of postoperative liver metastasis of gastric cancer. Methods Human gastric cancer and matched normal gastric tissues were collected, and SH3PXD2A - AS1 was detected by fluorescence quantitative realtime polymerase chain reaction （PCR）. The relationship between SH3PXD2A- AS1 expression and patient＇s sex, age, tumor location, tumor diameter, histological differ- entiation, depth of tumor invasion, lymph node metastasis, the number of liver metastases and the time of liver metastasis distant metastasis was analyzed, respectively. Results The positive rate of SH3PXD2A - AS1 in 37 cases of gastric cancer was 91.89% （34/37） , and that in normal gastric tissues was 5.40% （2/37）. The difference was very significant （x^2 = 55. 392, P = 0.000）. SH3PXD2A - AS1 expression was not significantly correlated with the age （x^2 =0. 560,P =0. 756） and sex （x^2 = 1. 718,P =0. 190）, tumor location （x^2 = 2. 839, P = 0. 242 ）, histological differentiation （x^2 = 0. 924, P = 0. 336 ）, but with the pri- mary tumor diameter （x^2 = 4. 788,P = 0. 029）, depth of tumor invasion （x^2 = 8. 815, P = 0. 012）, lymph node metastasis （x^2 = 6. 801,P = 0. 009）, the number of liver metastases （x^2 = 10. 157, P = 0. 001 ）, and liver metastasis time （x^2 = 6. 903, P = 0. 032）. Conclusion SH3PXD2A - AS1 has important predictive value in the diagnosis of postoperative liver metastasis of gastric cancer.