急性创伤应激对小鼠行为变化的影响

Effect of acute traumatic stress on behavioral changes in mice

目的研究急性创伤应激对小鼠自发行为、焦虑、学习记忆的影响。方法将16只小鼠分为实验组(n=8)和对照组(n=8),实验组给予足底电击,对照组不给予任何处理;分别在处理后0 h、12 h、7 d进行旷场实验以及高架十字迷宫实验,以评价小鼠行为学改变;用水迷宫实验以评价小鼠学习记忆改变。结果 (1)两组小鼠的站立次数和中央区域活动时间、路程在应激后0 h和12 h差异均有统计学意义(P<0.01),实验组少于对照组;应激后7 d两组仅中央活动时间差异有统计学意义(P<0.05);(2)实验组小鼠进入开臂次数及停留时间在应激后立即、12 h较对照组均显著减少(P<0.05);(3)实验组小鼠第2、3天的逃避潜伏期较对照组显著延长(P<0.05),穿越平台次数及目标象限游程比例较对照组均显著增多(P<0.05)。结论足底电击可引起小鼠自发行为减少,导致探索样行为减弱,焦虑增加,学习能力降低,但在一定程度上增强小鼠的空间记忆能力。

Objective To determine the effect of acute traumatic stress on spontaneous behaviors, anxiety, learning and memory in mice. Methods Sixteen mice were randomly divided into experimental group （n = 8 ） and control group （n = 8 ）. The mice of experimental group were given plantar shock while those of the control group were not. At the initial time after plantar shock （0 h） , and in 12 h and 7 d after the infliction, open field test and elevated plus maze test were used to observe the behavior changes, and water maze test was employed for the changes of learning and memory. Results （1）The standing time and the central activity time and distance showed significant differences between the 2 groups in 0 and 12 h after stress （P 〈0.01 ） , with the experimental group less than control group, but significant difference was observed in the central activity time on the 7th day （ P 〈 0.05 ）. （2）The number of enter open arms and residence time in open arms were significantly reduced in the mice from the experimental group than the control group at immediate and 12 h after stress （ P 〈 0. 05 ）. （3）The escape latency was significantly prolonged in the experimental group than the control group on the 2nd and 3rd days （ P 〈 0.05 ） , and the crossing platform times and target quadrant run ratio were also obviously larger than control group （P 〈 0.05）. Conclusion Plantar electric shock can lead the decrease of spontaneous behavior in mice, decrease the exploratory behavior and learning ability, and increase the anxiety behavior, and to some extent, enhance spatial memory ability.