髓样分化因子88基因rs7744多态性与冠状动脉粥样硬化性心脏病易感性及严重程度的关系

Relationship of MyD88 rs7744 Polymorphism with the Risk and Severity of Coronary Artery Disease

目的探讨中国北方汉族人群髓样分化因子88(MyD88)基因3’非编码区(3’-UTR)rs7744多态性与冠状动脉粥样硬化性心脏病(CAD)发病风险及严重程度的关系。方法收集2013年9月至2015年9月中国医科大学附属第一医院血液标本810例。CAD组540例,对照组270例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测rs7744多态性,并收集临床资料。结果 MyD88基因rs7744符合Hardy-Weiberg平衡(P>0.1)。对MyD88基因rs7744多态性与CAD发病风险的关系进行总体和分层分析。MyD88基因rs7744多态位点,年龄<50岁,AG突变杂合型携带者CAD的发病风险是AA野生纯合型携带者的0.38倍(95%CI:0.17~0.93;P=0.029)。对My D88基因rs7744多态性与CAD严重程度的关系进行冠状动脉病变支数及改良Gensini评分分析。在改良Gensini评分分析中,My D88基因rs7744多态位点,GG突变纯合型携带者的改良Gensini评分显著低于AA野生纯合型携带者(5.23±3.85 vs 7.49±4.96;P=0.011)。结论 MyD88基因3’-UTR区rs7744多态性与CAD发病风险及严重程度存在关联关系。携带rs7744突变基因型的年龄<50岁人群,CAD的发病率比较低。rs7744突变基因型携带者的改良Gensini评分显著低于野生型携带者。

Objective To investigate the association between rs7744 polymorphism in the 3＇ -untranslated region （3＇ -UTR） of myeloid differen- tiation factor 88 （MyD88） gene and the risk and severity of coronary artery disease （CAD） in a North Chinese Han population. Methods The CAD and control groups consisted of 540 patients and 270 subjects, respectively. The genotypes of rs7744 were detected by polymerase chain reac- tion-restriction fragment length polymorphism （PCR-RFLP）, and clinical data were collected. Results The distributions of MyD88 rs7744 were in HWE （P 〉 0.1 ）. In the 〈 50-year-old age group, a lower risk of CAD was observed for subjects carrying the variant AG genotype in comparison to subjects carrying the wild AA genotype （ OR = 0.38,95% CI ： 0.17-0.93, P = 0.029）. The MyD88 rs7744 polymorphism was also related to the modified Gensini score （P = 0.011 ）, which was lower in subjects carrying the variant GG genotype than the wild AA genotype （5.23±3.85 vs 7.49±4.96）. Conclusion Our results revealed that MyD88 rs7744 polymorphism in the 3＇ -UTR is correlated with the risk and severity of CAD.