姜黄素联合顺铂对人舌鳞癌Tca-8113细胞的影响

Effect of Curcumin Combined with Cisplatin on Human Tongue Squamous Cell Carcinoma Tca-8113 Cells

目的姜黄素或姜黄素联合顺铂在体外作用于人舌鳞癌Tca-8113细胞,观察其对人舌鳞癌细胞增殖的影响,并探讨可能的抗肿瘤作用机制。方法将培养的Tca-8113细胞随机分为姜黄素组、顺铂组、姜黄素联合顺铂组和对照组。MTT法检测各组药物对舌鳞癌Tca-8113细胞增殖的抑制作用;Hoechst 33258染色法观察各组药物对舌鳞癌细胞核的形态影响;流式细胞术检测细胞凋亡率;Western blotting检测各组药物对舌鳞癌Tca-8113细胞Notch1和表皮生长因子受体(EGFR)蛋白表达的影响。结果 MTT法结果显示,姜黄素联合顺铂组抑制舌鳞癌Tca-8113细胞增殖的作用明显高于姜黄素组和顺铂组(P<0.05)。流式细胞分析结果显示,姜黄素联合顺铂组的细胞凋亡率明显高于姜黄素组和顺铂组(P<0.05)。Western blotting结果显示,与姜黄素联合顺铂组比较,姜黄素组和顺铂组Notch1表达明显上调,EGFR表达则明显下调,而姜黄素联合顺铂组对Notch1和EGFR表达调控的影响与姜黄素组和顺铂组相比差异有统计学意义(P<0.05)。结论姜黄素与顺铂在抑制人舌鳞癌Tca-8113细胞增殖时具有协同效应,其机制可能与上调Notch1信号通路、下调EGFR信号通路有关。

Objective To observe the effect of curcumin combined with cisplatin on the proliferation of human tongue squamous cell carcinoma Tea-8113 cells, and explore the possible mechanism of their anti-tumor effect in vitro. Methods In this study, the samples were divided into four groups ： curcumin, cisplatin, cureumin combined with cisplatin, and control. Detection of rate of inhibition of Tca-8113 cell proliferation was carried out by MTT assay. This was followed by observation of the morphological changes of the nuclei by Hoechst 33258 fluorescence staining. Subsequently, cellular apoptosis was assayed by flow cytometry, and expression of Notchl and epidermal growth factor receptor （EGFR） was examined by Western blotting. Results Results of MTF assay showed that cureumin combined with cisplatin inhibited the proliferation of tongue squamous cell carcinoma to a greater extent than either curcumin or cisplatin alone （P 〈 0.05）. Flow cytometry analysis indicated that, unlike curcumin or cisplatin alone, curcumin combined with cisplatin noticeably induced apoptosis （P 〈 0.05）. Results of Western blotting revealed that the expression of Notchl was upregttlated, whereas the expression of EGFR was downregulated to a greater extent in the control group than in cells treated with cureumin or cisplatin alone. Unlike curcumin and cisplatin groups, the combined group revealed statistically significant expressions of Notch1 and EGFR （P 〈 0.05）. Conclusion Curcumin and cisplatin have a combined effect on inhibition of proliferation of human tongue squamous cell carcinoma Tca-8113 cells. The mechanism may be related to upregulation of the Notchl signaling pathway and downregulation of the EGFR signal- ing pathway.