武隆猪腰枣的组织培养研究

Tissue Culture of Jujube (Zizyphus jujuba) Mill.cv.Wulongzhuyaozao

以武隆猪腰枣为试材,以茎尖、带芽茎段、节间和叶片为外植体,开展了组织培养研究.结果表明,外植体以带芽茎段最好;适宜的初代培养基以1/2MS+1mg/L 6-BA+0.2mg/L NAA,接种的带芽茎段萌芽快,其愈伤组织的不定芽再生率达53.3%;最佳不定芽继代培养基为MS+1mg/L 6-BA+0.2mg/L IBA,增殖系数达3.77;不定芽在1/2MS+0.3mg/L IBA和1/2MS+0.3mg/L IBA+10g/L AC培养基上生根效果较好,生根率达90%~96.67%.

The shoot-tips, mature stem segments with buds, internodes and leaves were used as explants in a study of the tissue culture of jujube （Zizyphus jujuba） Mill. cv. Wulongzhuyaozao. The mature stem seg- ments with buds were shown to be the most suitable explants. In the primary culture, 1/2MS＋1 mg/L 6-BA＋ 0.2 mg/L NAA was the best medium, in which the bud sprouted fast, and the rate of adventitious shoot regeneration from the induced calli was 53.3%. The best medium for proliferation was MS＋1 mg/L 6-BA＋0. 2 mg/L IBA, and the bud multiplication coefficient was as high as 3.77. The adventitious shoots rooted readily on 1/2MS＋0.3 mg/L IBA＋10 g/L activated carbon, with a rooting percentage of 90%-96.67%. The identification of suitable conditions for tissue culture of Wulongzhuyaozao laid a good foundation for virus elim ination, rejuvenation and variety improvement of this jujube cultivar.