山楂沙棘片对小鼠通便功能实验研究

Effect of Hawthorn and Sea-buckthorn Tablet on Defecation Function in Mice

研究山楂沙棘片对小鼠排便功能及小肠运动能力的影响。试验随机分为空白对照组、模型对照组、阳性对照组、山楂沙棘片高剂量组、山楂沙棘片中剂量组、山楂沙棘片低剂量组,且都连续灌胃给予受试物7d,每日1次,末次灌胃受试物后禁食不禁水。排便实验禁食20h后给予洛哌丁胺10mg/kg,0.5h后再灌胃含相应受试物的墨汁,测定排出首粒黑便的时间以及排便粒数和重量;小肠运动实验禁食20h后给予洛哌丁胺5mg/kg,0.5h后再灌胃含相应受试物的墨汁,精确计时25min后脱椎处死,计算墨汁推进率。山楂沙棘片高剂量组、中剂量组与模型对照组相比,能显著缩短排出首粒黑便时间、显著增加排便粒数及重量、显著提高墨汁推进率。山楂沙棘片对提高通便功能有明显效果。

To observe the effect of Hawthorn and Sea-buckthorn Tablet （HST） on defecation function and small intestine movement func- tion in mice. Experiment of defecation and mall intestine movement＇s rats were both randomly divided into six groups which were control group, model group, positive group, HST high-dose group, HST middle-dose group and HST low-dose group. All the groups of two ex- periments were both given by gavage once a day for7 days. After last time ofgavage administration, all the group were fasting for solid. The defecation function experiment＇s groups were given loperamide 10mg/kg by gavage administration after fasting for 20 hours. Waiting for 30 minutes, all the group were given carbo medicinalis which contains corresponding test sample by gavage administration. Deter- mined the time of defecating first black stool, the particle numbers and weights of stool. The small intestine movement experiment＇s gro- ups were given loperamide 5mg/kg by gavage administration after fasting for 20 hours. Waiting for 30 minutes, all the group were given carbo medicinalis which contains corresponding test sample by gavage administration. Execute the rats after precise timing 25minutes, calculate the carbo medicinalis pro pulsive rate. Compared with model group, HST high-dose and middle-dose groups could shorten the time of defecating first black stool and increase the particle numbers and weights of stool obviously, enhance the carbo medicinalis pro pulsive rate obviously. HST has an evident effect on defecation function.