摘要
本实验建立了同时测定杞明片中5种蒽醌的方法。色谱柱:Diamonsi LC18(4.6mm 250mm,5 m);流动相:乙腈-0.1%磷酸溶液,梯度洗脱(0min^20min,40∶60;20min^30min,60∶40;30min^50min,90∶10);检测波长:284nm;流速:1.0m L/min;柱温:25℃。橙黄决明素在0.001 36mg/m L^0.006 80mg/m L、大黄酸在0.000 65mg/m L^0.003 25mg/m L、大黄素在0.000 13mg/m L^0.000 65mg/m L、大黄酚在0.000 96mg/m L^0.004 80mg/m L、大黄素甲醚在0.000 54mg/m L^0.002 70mg/m L范围内呈良好的线性关系;精密度、稳定性、重复性试验的RSD<1%;平均回收率分别为99.73%、99.53%、99.31%、99.69%、99.33%。所用方法简便可行、重现性好、准确度高,可作为同时测定杞明片中5种蒽醌含量的方法。
This experiment was established for the simultaneous determination of five kinds of free anthraquinone methods of Qiming tab- lets.Chromatographic column is DiamonsiL Cls （4.6mm×250mm, 5μm）, acetonitrile -0.1% phosphoric acid solution as mobile phase, gradient elution （0min-20min, 40 ： 60： 20min-30min, 60 ： 40： 30min-50min, 90 ： 10）, the detection wavelength of284nm 1.0mL/min, flow rate, column temperature of 25℃.The linear ranges were in concentration of 0.001 36 mg/mL -0.00 680mg/mL for chrysophanol, in concentration of 0.000 65mg/mL-0.003 25mg/mL for emodin, in concentration of 0.000 13mg/mL-0.000 65mg/mL for physcion, in concentration of 0.000 96mg/mL-0.004 80mg/mL for obtusifolin, in concentration of 0.000 54 mg/mL-0.002 70mg/mL for aurantio-ob- tusin, in precision and stability; repeat the test, RSD 〈 1%; the average recovery rate was 99.73%, 99.53%, 99.31%, 99.69%, 99.33%. the method is simple and feasible, of good reproducibility and high accuracy, which can be used as a method for simultaneous determi- nation of five kinds of anthraquinone in the Qiming tablets.
出处
《特产研究》
2017年第2期59-62,共4页
Special Wild Economic Animal and Plant Research
基金
吉林省医药健康产业发展引导资金(201603075YY)
