摘要
Abstract The metabolites of tall fescue leaves under normal and drought conditions were analyzed with LC-ESI-MS ( liquid chromatography tandem mass spec- trometry). A total of 282 metabolites were stably detected in the two groups using OPI.S-DA (orthogonal partial least squares discriminant analysis) method; 148 of which were down-regulated while 134 were up-regulated. After analyzed with MZ-minc software, 10 major metabolites (4 down, 6 up) were identified through searehing public database. Aromatic compounds, 3-p-eoumaroyll, 5-quinolaetone, aromatic free amino acids, styraxin mad methanofuran, were up-regulated ; grease metabolites, methyl 15-hydroperoxy-9Z, 12Z, 16E-octadecatrienoate, 9,12,13-Tri-HOME (10), 3-oxo-dodecanoic acid, and 13 (S)-HpOTrE acid ester, were down-regulated; piperanine (alkaloids) was down-regulated while Methyl 7-epi-12-hydroxyjasmonate glucoside (indicant) was up-regulated. In a word, aromatic compounds and liquid metabolites may play vital roles in drought-resistant regulation of tall fescue.
Abstract The metabolites of tall fescue leaves under normal and drought conditions were analyzed with LC-ESI-MS ( liquid chromatography tandem mass spec- trometry). A total of 282 metabolites were stably detected in the two groups using OPI.S-DA (orthogonal partial least squares discriminant analysis) method; 148 of which were down-regulated while 134 were up-regulated. After analyzed with MZ-minc software, 10 major metabolites (4 down, 6 up) were identified through searehing public database. Aromatic compounds, 3-p-eoumaroyll, 5-quinolaetone, aromatic free amino acids, styraxin mad methanofuran, were up-regulated ; grease metabolites, methyl 15-hydroperoxy-9Z, 12Z, 16E-octadecatrienoate, 9,12,13-Tri-HOME (10), 3-oxo-dodecanoic acid, and 13 (S)-HpOTrE acid ester, were down-regulated; piperanine (alkaloids) was down-regulated while Methyl 7-epi-12-hydroxyjasmonate glucoside (indicant) was up-regulated. In a word, aromatic compounds and liquid metabolites may play vital roles in drought-resistant regulation of tall fescue.
基金
Supported by Special Foundation of Guizhou Academy of Agriculture Science"Germplasm Innovation and Application of Drought and Heat Resistant Alfalfa"(QNKYYZX[2013]003)
Special Breeding Project of Guizhou Agriculture Commission"Cultivation of Flowering-inhibitory Tall Fescue Varieties with RNA Interference Technique"(QNYZZ[2013]012)