生殖支原体感染与女性黏液脓性宫颈炎相关性研究

Study on the relationship between Mycoplasma genitalium infection and female mucopurulent cervicitis

目的建立生殖支原体Taqman MGB荧光聚合酶链反应(PCR)检测方法,检测宫颈炎患者生殖支原体感染情况,以期发现生殖支原体感染与女性黏液脓性宫颈炎之间的相关性。方法参照国内、外文献建立Taqman MGB荧光PCR检测技术;采集大连市皮肤病医院性病门诊有黏液脓性宫颈炎的女性患者生殖道分泌物标本74例、体检中心对照组标本63例,应用上述检测方法检测标本中的生殖支原体。结果 Taqman探针荧光PCR法敏感性和特异性均较好。黏液脓性宫颈炎患者生殖支原体阳性率为24.3%,对照组未检测出生殖支原体;除不孕以外其他几项调查内容包括尿道炎症状、非婚性接触史、复发性宫颈炎以及治疗史均与生殖支原体感染有关联(P<0.001),其阳性率分别为17.6%、52.9%、42.5%、100%。结论 Taqman MGB荧光PCR法是目前较为适用的生殖支原体检测方法。生殖支原体感染可能是黏液脓性宫颈炎的致病因素;生殖支原体的检测在指导复发性宫颈炎的治疗中有较为重要的意义。

Objective To establish Taqman MGB real time fluorescence-based PCR assay for the detection of Mycoplasma genitalium（M.genitalium）, and apply the method to evaluate the infection rate of M.genitalium in STD clinic patients who were suffering from cervicitis in our hospital, so as to find the correlation between M.genitalium infection and mucopurulent cervicitis（MPC）. Methods One kind of Taqman MGB real time PCR assay was established for the detection of M.genitalium referring to the studies. A total of 74 patients with non-gonococcal, non-chlamydial MPC and 63 healthy female volunteers were recruited in our study. Cervical samples were collected and M.genitalium was detected by Taqman MGB real time PCR assay. Results The results indicated that the Taqman MGB real time PCR had good linear range, great repeatability and High sensitivity, the lowest detectable limit was 5 copies. The positive rates of M.genitalium in the MPC patients was 24.3% while none M.genitalium was found in the control group. Significant difference was found in the both two groups（P〈0.001）. In addition to infertility, several other factors including urethritis symptoms, sexual contact history, recurrent cervicitis and treatment history were associated with mycoplasma infection（P〈0.001）, and the positive rate of M.genitalium was 17.6%, 52.9%, 42.5% and 100% respectively. Conculsion The Taqman MGB real time fluorescence based PCR is a rapid and sensitive method for the quantitative and qualitative detection of M.genitalium. The prevalence of M.genitalium infection is higher in patients with non-gonococcal, non-chlamydial mucopurulent cervicitis attending STD clinic than that in normal population. Detection of M.genitalium has a great significance in guiding the treatment of recurrent cervicitis.