拟南芥P5CS1基因转化羽衣甘蓝增强耐盐性分析

Salt tolerance strengthening of Brassica oleracea var. acephala f. tricolor genetically modified by P5CS1 gene from Arabidopsis thaliana

【目的】分析转拟南芥△1-吡咯啉-5-羧酸合成酶(P5CS1)基因羽衣甘蓝的耐盐性,为获得较强的耐盐性羽衣甘蓝品种及其抗逆育种提供理论依据。【方法】将拟南芥P5CS1基因(AtP5CS1)经农杆菌介导转入羽衣甘蓝植物中,在盐胁迫下,分别检测转基因植株与野生型植株的AtP5CS1 mRNA表达量、幼苗脯氨酸含量、株系根系性状、整株干质量和鲜质量、叶片相对水含量、叶片电导率和整株存活率。【结果】在150 mmol/L NaCl胁迫下,转基因植株的P5CS1基因mRNA可正常表达,与对照相比,转基因株系Y1、Y2的主根和最长侧根长度较长,侧根数目较多,整株干质量和鲜质量较重;而且相对水含量显著高于对照植株(P<0.05,下同),脯氨酸含量及存活率均极显著高于对照植株(P<0.01),叶片相对电导率显著低于对照植株。【结论】转AtP5CS1基因植株的耐盐表型优于对照,即AtP5CS1基因在羽衣甘蓝中的表达明显改善了转基因植株的耐盐性。

【Objective】Salt tolerance of Brassica oleracea var. acephala f. tricolor after being transferred with △1-pyrroline-5-carboxylate synthetase（P5CS1） gene from Arabidopsis thaliana was analyzed, in order to obtain slat-tolerant B. oleracea var. acephala f. tricolor varieties and provide reference for breeding for stress tolerance. 【Method】P5CS1 gene from A. thaliana was transferred into B. oleracea var. acephala f. tricolor via Agrobacterium. Under salt stress,expression of P5CS1 mRNA, proline content in seedlings, root traits, dry weight of whole plant,fresh weight of whole plant,relative water content in leaf, relative electric conductivity of leaf and whole plant survival rate in transgenic plants and wild plants were measured. 【Result】Under 150 mmol/L NaCl stress, P5CS1 mRNA of transgenetic plants was in normal expression. Compared with control, main roots and the longest lateral roots of transgenic plants Y1 and Y2 were long, lateral roots were abundant, dry weights and fresh weights of whole plant were heavy. Their relative water contents were significantly higher than that of control（P〈0.05, the same below）, proline contents and survival rates were extremely significantly higher than that of control（P〈0.01）, but relative electric conductivity of leaf was significantly lower than that of control. 【Conclusion】Salt tolerance of plants genetically modified by AtP5CS1 was better than control. AtP5CS1 gene expression significantly improves salt tolerance of transgenic plants in B. oleracea var. acephala f. tricolor.