基于多项式回归分析的摩尔多瓦葡萄富硒效应

Selenium enriched Moldova grape research based on polynomial regression analysis

【目的】探讨基于多项式回归分析的摩尔多瓦葡萄富硒效应,为开展广西富硒土原位生产富硒葡萄提供参考依据。【方法】以摩尔多瓦葡萄为试验材料,采用三元二次饱和D-最优设计,以石灰石粉(x_1)、AM菌剂(x_2)、营养调节剂(x_3)为因变量,以试验结果中葡萄硒含量为自变量(y),设11个处理(处理1~处理11),经回归分析明确试验因素对葡萄硒含量的影响,优选生产富硒摩尔多瓦葡萄的外源物质施用量。【结果】施用石灰石粉、AM菌剂和营养调节剂各处理(除处理1外)摩尔多瓦葡萄的硒含量比对照(CK)提高1.1~1.8倍,最高增幅达87.2%。多项式回归方程中单因素效应分析结果显示,当石灰石粉、AM菌剂和营养调节剂施用量分别为2400.00、69.00和39.98 kg/ha时,摩尔多瓦葡萄硒含量最高,为11.56μg/kg;互作效应分析结果显示,两两互作对摩尔多瓦葡萄硒含量的影响排序为x_1x_3>x_1x_2>x_2x_3。【结论】摩尔多瓦葡萄分别开沟施用石灰石粉、AM菌剂和营养调节剂2400.00、69.00和39.98 kg/ha,其果实硒含量达富硒水平。因此,施用石灰石粉、AM菌剂和营养调节剂等外源物质可作为生产富硒葡萄的新型技术手段。

【Objective】Selenium enriched Moldova grape was researched based on polynomial regression analysis, so as to provide reference for planting selenium enriched grapes in Guangxi selenium enriched field without adding selenium substance. 【Method】Moldova grape was used as the material in experiment. With ternary quadratic saturation D-optimum design,limestone powder（x1）,AM fungal inoculum（x2）, nutrient regulators（x3） as dependent variables, selenium content in grape as independent variable（y）, 11 treatments（treatment 1-11） were set to conduct regression analysis. Through the experiment, effects of experiment factors on selenium content in Moldova grape were studied, in order to determine application rates of allogenic materials for selenium enriched grapes. 【Result】In all treatments applying limestone powder, AM fungal inoculum and nutrient regulators（except treatment 1）, selenium contents in Moldova grape increased 1.1 to 1.8 times compared with CK, the maximum increasement reached 87.2%. Single factor effect results showed that selenium content in grapes was the highest when applying 2400.00 kg/ha limestone powder, 69.00 kg/ha AM fungal inoculum and 39.98 kg/ha nutrient regulators, and the selenium content in Moldova grape was 11.56 μg/kg. Interaction effects analysis indicated that effects rank of two factors interaction on Moldova grape were as follows： x1x3〉x1x2〉x2x3. 【Conclusion】Applying 2400.00 kg/ha limestone powder, 69.00 kg/ha AM fungal inoculum and 39.98 kg/ha nutrient regulators to Moldova grape plants, the fruits can reach selenium enriched level. Therefore, application of limestone powder, AM fungal inoculum and nutrient regulators can be utilized as a new technique for planting selenium enriched grapes.