载阿霉素pH敏感磁性纳米粒的合成及表征

Preparation and Characterization of Doxorubicin-loaded pH/magenetic Dual-responsive Nanoparticles

目的制备具有pH敏感的磁性阿霉素(Doxorubicin,DOX)纳米粒Fe_3O_4@nSiO_2@mSiO_2@PEG-b-PAsp@DOX并进行表征及优化。方法利用透射电镜、磁滞回线测试仪等对其进行表征,设计正交试验筛选最佳载药条件。结果载体材料的粒径约为200nm,电位值为-36.5m V,纳米粒的磁饱和强度为8.03emu·g^(-1),确定纳米粒的最佳合成条件为载体材料10mg,高分子4mg,阿霉素4mg,反应时间为1h,载药量高达(58.50%±3.91%),在pH 5.2环境下累积释放量较pH 7.4高。结论所制得的Fe_3O_4@nSiO_2@mSiO_2@PEG-b-PAsp@DOX载药量高,且具有pH敏感特性及超顺磁性。

OBJECTIVE To prepare doxorubicin-loaded pH/magenetic dual responsive nanoparticles denoting as Fe304@ nSiOz @ mSiO2@ PEG-b-PAsp@ DOX. Determining its properties and optimizing the prepara- tion. METHODS Physicochemical properties were determined by transmission electron microscopy,hysteresis loop tester and so on. The orthogonal test design was used to optimize the preparation of nanoparticles. RESULTS The average particle size and the zeta potential of carrier material were 200nm and -36.5mV respectively. The value of saturated magnetic of nanoparticles was 8.03emu · g^-l. The best synthesis conditions of nanoparficles are was fol- lows ：the carrier dosage was 10rag, the polymer 4mg, the doxorubicin 4mg and the reaction time was l h. It＇s drug loading up to （58.50% ± 3.91% ）and cumulative release rate at pH 5.2 environment was higher than at pH 7.4. CONCLUSION Fe304@ nSiO2@ mSiO2@ PEG-b-PAsp@ DOX we made possess high drug loading,pH-sensi- tire nronerties and superparamagnetic.