正交试验法优化复方苍夷胶囊提取工艺研究

Studies on the Extraction Technology of Fufang Cangyi Capsule with Orthogonal Test

目的应用正交试验法优选复方苍夷胶囊的最佳提取工艺。方法以方中辛夷中木兰脂素和黄芩中黄芩苷含量为考察指标,用正交试验法优选提取乙醇浓度、提取时间、药液比等提取参数。结果最佳提取工艺为:取辛夷粗粉、薄荷、金银花及连翘,加入10倍量水,浸泡12h,回流提取6h,收集挥发油和蒸馏后水溶液;药渣与苍耳子、黄芩等其余8味药用70%乙醇浸泡过夜,以浓度为70%的乙醇作为溶剂,按照药液比1∶12的体积,提取2次,每次2h,合并2次提取液与蒸馏后的水溶液。结论经优选的提取工艺合理而可行。

OBJEVTIVE To optimize the extraction technology of Fufang Cangyi Capsule with orthogonal test. METHODS Based on the contents of magnolin in Magnolia denudata Desr, and baicalin in Scutellaria ba-icalensis, the amount of ethanol, extracting time and liquid ratio were optimized by the 0rthogonal test. RESLUTS The optimum condition of Fufang Cangyi Capsule was：Xinyi crude powder, Bohe, Jinyinghua and Lianqiao soaked with 10 times amount of water for 12 h, then refluxed extraction for 6 h, the volatile oil and distilled water solution were collected. The residue and Cangerzi, Huangqin and other 8 medicines soaked with 70% ethanol overnight, then extract with times amount 70% ethanol twice, each 2h, combined the extracts and distilled water. CONCLUSION The extraction technology optimized by orthogonal test is simple and reasonable.