电针联合氟西汀抑制慢性应激抑郁大鼠额叶皮质nNOS、c-Fos上调和SYP下调

Effects of electroacupuncture combined with fluoxetine on nNOS,c-Fos and SYP expression in the frontal cortex of chronic stress depression rats

目的:观察电针联合氟西汀对慢性应激抑郁模型大鼠额叶皮质神经元型一氧化氮合酶(nNOS)、基因片段(c-Fos)和突触素(SYP)表达的作用。方法:SD大鼠随机分为正常对照组、模型组、电针组、氟西汀组和电针+氟西汀组。对大鼠进行21 d的应激刺激建立慢性应激抑郁模型。按分组给予电针刺激百会、神庭穴30 min,氟西汀灌胃,每天1次,持续21 d,旷场实验检测大鼠行为学。免疫组织化学法检测额叶皮质nNOS、c-Fos和SYP的表达。结果:与正常对照组比较,模型组大鼠额叶皮质nNOS和c-Fos阳性神经元表达增加(P<0.05),SYP阳性神经元表达减少(P<0.05),大鼠行为学指标降低;与模型组比较,电针组、氟西汀组和电针+氟西汀组大鼠额叶皮质nNOS和c-Fos阳性神经元表达减少(P<0.05),SYP阳性神经元表达增加(P<0.05),大鼠行为学指标提高;与电针组、氟西汀组比较,电针+氟西汀组大鼠额叶皮质nNOS和c-Fos阳性神经元表达减少(P<0.05),SYP阳性神经元表达增加(P<0.05),大鼠行为学指标提高。结论:电针联合氟西汀可发挥协同作用,可能通过抑制慢性应激抑郁对额叶皮质nNOS、c-Fos的上调和SYP的下调,更好地达到抗抑郁的作用。

Objective：To observe the effects of electroacupuncture combined with fluoxetine on regulating the expressions of neuronal nitric oxide synthase（nNOS）,c-Fos and synaptophysin（SYP） in frontal cortex of depressed rats from chronic stress.Methods： SD rats were randomized into normal control group,model group,electroacupuncture group,fluoxetine group and electroacupuncture plus fluoxetine group,and subjected to stress stimulation for 21 days to establish chronic stress depression model.Then the rats were given electroacupuncture stimulation via acupoint at Baihui and Shenting for daily 30 min plus fluoxetine once per day for consecutive 21 days by groups.Open field test was performed to observe the behavior of the rats,and immunohistochemistry was used to detect nNOS,c-Fos and SYP expression in the frontal cortex of the experimental animals.Results： Compared with the normal control group,expression of nNOS and c-Fos-positive neurons was increased in the frontal cortex of rats in model group（P〈0.05）,yet the expression of SYP-positive neurons was decreased（P〈0.05） and scoring of behavior index was also decreased.Rats in electroacupuncture group,fluoxetine group and electroacupuncture plus fluoxetine group had decreased expression of nNOS and c-Fos-positive neurons and increased SYP-positive neurons in their frontal cortex,and had higher scores in behavioral index than those in the model group（P〈0.05）.By comparison with the electroacupuncture group and fluoxetine group,rats in the electroacupuncture plus fluoxetine group had decreased expression of nNOS and c-Fos-positive neurons and increased SYP-positive neurons in the frontal cortex,and improved behavioral index measurment（P〈0.05）.Conclusion： Electroacupuncture plus fluoxetine can produce synergistic effects on the neurons in the frontal cortex of rats,and lead to better antidepressant effect via inhibiting up-regulation of nNOS and c-Fos as well as down-regulation of SYP down-regulation.