HCV感染男性吸毒者血浆CCL19及CCL21浓度变化分析

Level diversifies of plasma CCL19 and CCL21 in male drug users infected HCV

目的:分析丙型肝炎病毒(Hepatitis C virus,HCV)感染男性吸毒者血浆CCL19及CCL21浓度变化。方法:采用定量ELISA法检测血浆CCL19和CCL21浓度,ELISA法检测血浆抗HCV,Real-time RT-PCR法检测HCV RNA,以国家哨点监测HCV感染的391名男性吸毒者为研究对象,60名健康男性为对照,分析不同抗HCV/HCV RNA状况男性吸毒者血浆CCL19和CCL21浓度变化。结果:391名男性吸毒者中,180名感染HCV,感染率为46.04%。未感染HCV男性吸毒组血浆CCL19及CCL21浓度较健康男性组升高(P≤0.001)。吸毒抗HCV(-)/HCV RNA(+)组血浆CCL19和CCL21浓度最低,与其他组差异明显(P≤0.05);吸毒抗HCV(+)/HCV RNA(-)组的最高,与健康男性组差异显著(P<0.05)。结论:吸毒可使男性血浆CCL19和CCL21浓度升高。血浆CCL19和CCL21浓度升高可能有助于HCV自发清除;血浆CCL19和CCL21浓度降低可能预示着病程持续转归较差。

Objective：To analyze the level diversifies of plasma CCL19 and CCL21 in the male drug users infected HCV.Methods：The plasma CCL19 and CCL21,anti-HCV and HCV RNA were detected by ELISA quantitation,ELISA qualitation and Real-time RT-PCR respectively.Compared with 60 healthy man,the level diversifies of plasma CCL19 and CCL21 in 391 male drug users conducted as part of HCV Surveillance Programme in Zhuhai were analyzed.Results：180 of 391 male drug users were infected HCV and the infection rate was 46.04%.The level of plasma CCL19 and CCL21 in the male drug users[anti-HCV（-）/HCV RNA（-） ]were higher than that in the healthy man（P≤0.001）.The level of plasma CCL19 and CCL21 in anti-HCV（-）/HCV RNA（＋）group was lower than that in the others（P≤0.05）.Compared with that in the healthy man,the level of plasma CCL19 and CCL21 in the drug abuse anti-HCV（＋）/HCV RNA（-） group had significant deviation（P〈0.05）.Conclusion：Drug abuse can heighten the level of plasma CCL19 and CCL21 in man.Increasing the level of plasma CCL19 and CCL21 may conduce to spontaneous HCV clearance.It may prognosticate that HCV infection will be persistent and have a bad consequence when the level of plasma CCL19 and CCL21 were degraded.