LC-MS/MS鉴定大鼠血浆中桃红四物汤有效成分

Identification of Effective Constituents of Taohong Siwu Decoction in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry

目的研究桃红四物汤有效成分体内作用的物质基础。方法利用液相色谱-串联质谱(liquid chromatography technique coupled with tandem mass spectrometry,LC-MS/MS)提供的色谱及离子碎片信息,对大鼠灌胃桃红四物汤中入血成分进行分析鉴定。结果通过保留时间比对、离子碎片解析等综合分析,鉴定桃红四物汤中6个入血成分(梓醇、藁本内酯、阿魏酸、芍药苷、芍药内酯苷和苦杏仁苷)均为原形成分,入血成分可能为桃红四物汤体内直接作用的有效成分。结论本实验确定了桃红四物汤经口服后可被胃肠道吸收入血的6个有效成分,为后期桃红四物汤的药物代谢动力学研究提供了参考依据。

Objective To investigate the material basis for the in vivo effect of the effective constituents of Taohong Siwu Decoction. Methods The chromatographic information and information on fragment ions provided by liquid chromatography technique coupled with tandem mass spectrometry were used for the analysis and identification of the constituents （into blood） of Taohong Siwu Decoction given by gavage in rats. Results Based on the analysis of retention time and fragment ions, a total of six constituents （into blood） of Taohong Siwu Decoction were identified, i.e., catalpol, ligustilide, ferulic acid, paeoniflorin, albiflorin, and amygdalin, and all of them were original constituents. Such constituents were possibly the effective constituents of Taohong Siwu Decoction with a direct in vivo effect. Conclusion This experiment identifies six effective constituents of Taohong Siwu Decoction which can be absorbed into blood by the gastrointestinal tract after oral administration, which provides a reference for future pharmacokinetic studies of Taohong Siwu Decoction.