甘草对大戟缓解小鼠肝癌腹水的影响

Effect of Combination of Glycyrrhizae Radix et Rhizoma and Euphorbia Pekinensis Radix in Resisting Hepatocellular Carcinoma ascites in Mice

目的观察不同比例的甘草-大戟配伍煎剂对肝癌腹水小鼠的干预作用。方法 120只昆明小鼠随机分为空白、模型、大戟与甘草不同配比(1∶0.5、1∶1、1∶2和1∶4)、大戟单煎和甘草单煎共8组,每组15只。除空白组外,其他组小鼠腹腔均接种制备肝癌腹水小鼠模型,并分别灌胃给药12d,模型组给予等量生理盐水。12d后检测体质量、腹围、腹水量和肝肾指数,筛选甘草对大戟抗肝癌腹水药效影响显著的配比条件,并采用Western Blot法分析肾组织的水通道蛋白-2(Aquaporin-2,AQP2)和肾加压素受体(Renal vasopressin receptors-2,V2R)的蛋白含量,采用生化法测定小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、尿素氮(BUN)和尿肌酐(Cr)的含量。结果与模型组比较,大戟单煎组和大戟∶甘草1∶2组的腹水量、腹围和体质量显著减少(P<0.05~0.01),并降低肾组织AQP2和V2R的表达(P<0.05~0.01),其中,大戟∶甘草1∶2组能增强大戟单煎组的作用(AQP2:P<0.05,V2R:P<0.01),而大戟∶甘草1∶4组则降低单煎组的作用(AQP2:P<0.05,V2R:P<0.01)。结论大戟防治小鼠肝癌腹水的作用可能会因不同比例甘草的配伍而改变:或增强或被抵消,且相关变化可能与其有效调节了肾组织内的AQP2和V2R蛋白表达有关。

OBJECTIVE To investigate the effect of the combined administration of herbal pair Euphorbia Pekinensis （DJ） and Glycyrrhiza glabra （GC） at different doses or ratios on hepatocellular carcinoma （HCC） ascites in mice. METHODS A total of 120 male Kunming mice were randomly divided into normal control, model, DJ-GC groups [ratios 1 ： 0.5, 1 ： 1, 1 ： 2, 1 ： 4], DJ-alone group and GC-alone group. HCC ascites model was constructed by inoculating H22 cells intraperitoneally. HCC ascites mice in the drug treatment groups were intragastrically administrated with corresponding doses of Euphorbia Pe- kinensis and Glycyrrhiza glabra for 12 d, respectively. Then, the therapeutic effects of herbal pair DJ and GC acting on HCC acites was analyzed in terms of body weight, ascites volume, abdominal circumference and the index of liver and kidney. After that,the expressions of AQP2 and V2R were detected by Western Blot, and the level of ALT, AST, BUN, CR were detected by biochemical method. RESULTS When compared with model group, the DJ-alone group and DJ-GC 1 ： 2 group could effi- ciently reduced the ascites volume, abdominal, body weight （P〈0.05-0.01） and the expression of aquaporin-2 and renal vas- opressin receptors-2 in the kidney tissues （P〈0.05-0.01）. DJ-GC 1 ： 2 group could enhance the effect of DJ-alone group （ P 〈0.05-0.01 ）, while the DJ-GC 1：4 group could reduce the effect of DJ-alone group （ P〈0.05-0.01 ）. CONCLUSION The therapeutic effects of DJ on HCC were altered in accordance with its combination with GC at different ratios by being en- hanced or weakened, which might be partially related to their regulation on aquaporin-2 and renal vasopressin receptors-2 signal