基于AMDIS和保留指数结合的GC-MS技术定性鉴别醋炙前后香附-艾叶药对中挥发油化学成分的变化

Qualitative Investigation on Changes of Chemical Constituents from Volatile Oils Between Crude and Vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium Herb-pair Based on AMDIS and Retention Index Coupled with GC-MS Analysis

目的从药对的整体角度切入,以调经止痛的功效为评价模型,来探究醋炙对香附-艾叶药对整体配伍的影响。方法采用GC-MS结合AMDIS与保留指数鉴别方法并联合主成分分析寻找共性规律的研究策略,全面系统地分析了醋炙前后香附-艾叶药对中挥发油化学成分的变化。结果香附-艾叶(生品)挥发油中共鉴别出54种化学成分,而香附-艾叶(醋品)挥发油中共鉴别出52种化学成分。醋炙前后的香附-艾叶药对与醋炙前后的单味药中挥发油化学成分的变化并不一致。从整体来看,在醋香附-醋艾叶药对的挥发油中,多数低沸点化合物的含量降低,而高沸点化合物无明显变化,且大部分低沸点化合物主要归属于艾叶,大部分高沸点化合物主要归属于香附,α-香附酮的相对含量也有所增加。在醋香附-醋艾叶药对的挥发油中含量明显增加的化合物多为去氧倍半萜类化合物、长链脂肪醇和长链脂肪酮,而萜类氧化衍生物的含量则有所降低。结论采用GC-MS结合AMDIS与保留指数鉴别方法并联合主成分分析寻找共性规律的研究策略,可全面、科学和快速地鉴别醋炙前后香附-艾叶药对中挥发油的差异。从整体上看,醋炙使得香附-艾叶药对中挥发油的毒性减小,安全性增加,稳固并小幅增强了调经止痛的功效。

OBJECTIVE The effects of vinegar-processing on overall compatibility of Cyperi Rhizozna-Artemisiae Argyi Folium herb-pair were investigated based on assessment model of regulation on painful menstruation as efficacy from overall angle of the herb-pair. METHODS The experimental strategy to look for common regularity of GC-MS technology combined with principal component analysis and identification methods based on AMDIS and retention index was used to analyze the changes of chemical constituents from volatile oils between crude and vinegar-processed Cyperi Rhizorna-Artemisiae Argyi Folium herb-pair comprehensively and systematically. RESULTS Fifty-four chemical components had been identified in vola- tile oils of crude Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair while fifty-two chemical components had been identified in volatile oils of vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair. In addition, The changes of chemical constituents from volatile oils in crude and vinegar-processed Cyperi Rhizoma-Arternisiae Argyi Folium herb-pair were incon- sistent with those in crude and vinegar-processed Cyperi Rhizoma （Artemisiae Argyi Folium） . On the whole, in volatile oils of vinegar-processed Cyperi Rhizoma-Arternisiae Argyi Folium herb-pair, the content of most low boiling point compounds belonging to Artemisiae Argyi Folium was decreased while the content of most high boiling point compounds belonging to Cy- peri Rhizoma had no obvious change, and the relative content of a-cyperone was increased to some extent. The compounds with significantly increasing contents in volatile oils of vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair were deoxy-sesquiterpenoids, long chain fatty alcohols, and long chain aliphatic ketones. Nevertheless the content of terpenoid oxide derivatives was decreased. CONCLUSION By employing the strategy to look for common regularity of GC-MS technolo- gy combined with principal component analysis and identification methods based on AMDIS and retention index, we could iden- tify the differences of chemical constituents from volatile oils between crude and vinegar-processed Cyperi Rhizorna-ArtemisiaeArgyi Foliurn herb-pair comprehensively, scientifically, and rapidly. Overall, the toxicity of volatile oils in Cyperi Rhizorna- Artemisiae Argyi Folium herb-pair was decreased, the safety was increased, and the efficacy of regulation on painful menstru- ation was strengthened after vinegar-processing. The strategy used in this paper would be a certain reference significance for analyzing other complex systems on Chinese herbal medicines.