10批次伪品沉香鉴别方法的相关性

Correlation of Identification Method for 10 Batches Falsified Agarwood

【目的】针对目前沉香市场真假混杂、难以鉴别的现状,采用多种方法对10批次伪品沉香进行鉴别,分析各方法之间的相关性及可替代性,探究沉香真伪鉴别的有效手段,为沉香产业的健康发展提供帮助,为识别伪制沉香、完善沉香的质量标准提供依据。【方法】采用《药典》(2015版)中规定的醇溶性浸出物含量、显色反应、薄层色谱分析以及具有专属性分辨2-(2-苯乙基)色酮类成分的高效液相色谱(HPLC)分析沉香鉴别方法,对10批次伪品沉香进行鉴别,并通过对比8批次正品人工沉香样品和对照样品,探索各方法的准确性以及相关性。【结果】在10批次伪品沉香样品中,其醇溶性浸出物含量均达到要求;显色反应对颜色的判断具有强烈的主观性;薄层色谱分析鉴别具有专属性,但受沉香成分多样性、含量不一等因素影响,部分样品判别荧光斑点不清晰;HPLC方法制样简单,节省原料,可有效避免人为主观因素影响,能够对复杂沉香成分进行分离,通过对照指纹图谱6个特征峰和4个辅助峰,判断结果精确度高。【结论】各方法之间相辅相成,互相佐证,考虑到沉香样品检测过程需降低时间和原料成本,基于各方法的优势及精准程度,建议在沉香真伪鉴别中采用以醇溶性浸出物含量、HPLC指纹图谱结合显微鉴别为主,显色反应和薄层色谱分析为辅的方法。

[ Objective] In view of the present status of agarwood market, it is difficult to distinguish and identify the real agarwood. This research used a variety of methods to identify 10 batches fake agarwood, analyzed the correlations and fungibilities among the various methods, and then explored the effective ways of agarwood authenticity. Preliminary establishing a method for the identification of＇agarwood will provide effective help for the healthy development of agarwood industry, and improve the quality standards of agarwood authenticity. [ Method ] Using specified agarwood identification methods in the pharmacopoeia, including alcohol soluble extract content, color reaction, thin layer chromatography（TLC） analysis methods, and the specificity method to distinguish 2-（2-phenylethyl） chromones named high performance liquid chromatography（HPLC） , 10 batches falsified agarwood were respectively identified, and the accuracy and correlation of each method were compared between 8 batches authentic and control agarwood. [ Result] In 10 batches falsified samples, the soluble extract content were all suitable for the requirements, and the color reaction showed strong subjectively. TLC had specificity but it was affected by the diversity and content complexity of agarwood, which led to the discriminant fluorescence spots not clear. HPLC method was good at the separation of complex composition of agarwood. The identification results exhibited high precision by contrast fingerprint with six characteristic peaks and four secondary peaks. [ Conclusion] Each method supplements and supports each other. Considering both the time and rare sample cost, and the advantage and accuracy of the above mentioned methods, the agarwood identification was given priority to choose the HPLC fingerprint combining with microscopic identification. The color reaction and the TCL could be complementary.