摘要
目的:观察旋小檗碱对APP/PS1/Tau三转基因阿尔茨海默病(AD)小鼠海马组织β-淀粉样蛋白(Aβ)和Bcl-2、Bax表达的影响。方法:20只三转基因AD小鼠随机分为对照组和给药组,各10只。加药组给予小檗碱50 mg·kg^(-1)·d^(-1)灌胃,对照组给予等容量生理盐水灌胃,共3个月。Western Blot检测小鼠海马组织蛋白Aβ、Bcl-2和Bax表达,免疫组化测定小鼠海马Aβ表达,ELISA检测小鼠海马Bcl-2、Bax表达。结果:给药组海马组织Aβ表达较对照组降低(P<0.05);与对照组比较,给药组海马组织Bcl-2表达增加而Bax表达减少(P<0.05)。结论:小檗碱能降低三转基因AD小鼠的Aβ表达,其机制可能与Bcl-2和Bax表达改变有关。
Objective： To observe the effect ofberberine on the expression of amyloid-β （Aβ）, Bcl-2 and Bax in the hippocampus ofAPP/PS1/Tau threetransgenic mice ofAlzheimer＇ s disease （AD）. Methods： Twenty APP/ PS1/Tau three transgenic AD （3XTg-AD） mice were randomly divided into control group and treatment group （n=10 respectively）. Mice in treatment group were treated with berberine at the dose of 50 mg·kg^-1· d^-1 by gastric gavage orally for 90 days. Mice in control group were treated with the normal saline at the same dose by gasteic garage orally for 90 days. The Aβ, Bcl-2 and Bax protein in hippocampus were detected by western blot. The ex- pression of Aβ in the hippocampus was determined by immunohistochemistry. The Bcl-2 and Bax protein in hip- pocampus was detected by ELISA. Results： Compared with the control group, the expression of Aβ in the hip- pocampus of the berberine group was significantly decreased （P〈0.05）; the expression of Bcl-2 was more inten- sive in hippocampus tissue of berberine group than that of control group （P〈0.05）, but the expression of Bax was the opposite （P〈0.05）. Conclusion： Berberine can decrease the expression of Aβ in the hippocampus of APP/ PS 1/Tau three transgenic AD mice, and up-regulating Bcl-2 expression and down-regulating Bax expression may play a role in the mechanism.
出处
《神经损伤与功能重建》
2017年第3期194-196,共3页
Neural Injury and Functional Reconstruction
基金
深圳市科技研发资金项目(No.JCYJ20150529112551484)
