液相色谱串联质谱法测定动物组织中17种β-兴奋剂残留

Determination of 17 β-agonists Residual in Animal Tissue by Liquid Chromatography-Tandem Mass Spectrometer

[目的]建立动物组织中β-兴奋剂多残留的液相色谱-串联质谱(LC-MS/MS)分析方法。[方法]样品经提取、固相萃取柱净化后采用LC-MS/MS进行检测分析,对样品前处理条件和质谱参数进行研究。[结果]17种β-兴奋剂的线性范围在0.5~50.0 ng/mL相关性良好,线性相关系数在0.999 88~0.995 70;2种加标水平回收率为63.20%~109.92%和62.99%~112.19%,相对标准偏差(RSD)为0.63%~11.50%和0.33%~8.93%。[结论]该方法稳定、灵敏度高、选择性好,测定结果令人满意,可在各食品检测机构推广应用。

[ Objective] A sensitive and rapid HPLC-MS/MS method was developed for the determination of Beta-adrenergic agonistsresidues in animal tissue. [ Method] Samples were subjected to extraction, followed by solid phase extraction clean-up before HPLC-MS/MS analysis. The pretreatment conditions and mass spectra parameters were studied. [ Result] The developed standard curves of 17 Beta-adrenergic agonists all displayed good linearity over the concentration range of 0.5 - 50.0 ng/mL, and coefficient correlation （ R2 ） between 0. 999 88 - 0. 995 70. Mean recovery rates of 6 replicates for Beta-adrenergic agonist in a negative pork sample spiked at two levels between 63.20% - 109. 92% ;62.99% - 112.19%, RSD was 0.63% - 11.50% and 0.33% - 8.93%. [ Conclusion] This method proved to be of stability, high sensitivity and good selectivity and provided satisfying determination results. Therefore, its application deserves to be popularized among various food testing laboratories.