黄芪多糖协同增强卡维地洛改善扩张型心肌病大鼠心功能研究

Astragalus polysaccharide＇s synergistic enhancement on Carvedilol＇s improvement effects on heart function of rat with dilated cardiomyopathy

目的研究黄芪多糖协同增强卡维地洛改善扩张型心肌病（dilated cardiomyopathy，DCM）大鼠心功能。方法将DCM模型Wistar大鼠分为未处理组（CON组）、卡维地洛组（CAR组）、黄芪多糖组（APS组）及卡维地洛联合黄芪多糖组（C＋A组），比较4组DCM模型大鼠左室射血分数（LVEF）、Tei指数、高敏C．反应蛋白（hs-CRP）、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1、IL巧、IL-8及CD3＋、CD4＋和CD8＋T淋巴细胞亚群的差异。结果C＋A组和CAR组DCM模型大鼠LVEF显著高于CON组和APS组（均P〈0．05），Tei指数显著低于CON组和APS组（均P〈0．05）；C＋A组DCM模型大鼠LVEF显著高于CAR组（P〈0．05），Tei指数显著低于CAR组（P〈0．05）。C＋A组和APS组DCM模型大鼠hs—CRP、TNF-α、IL-1、IL-6和IL-8均显著低于CON组和CAR组（均P〈0．05），CD3＋、CD4＋和CD8＋T淋巴细胞亚群均显著高于CON组和CAR组（均P〈0．05）；C＋A组DCM模型大鼠hs-CRP、TNF-α、IL-1、IL-6和IL-8均显著低于APS组（均P〈0．05），CD3＋、CD4＋和CD8＋T淋巴细胞显著高于APS组（均P〈0．05）。结论黄芪多糖可协同增强卡维地洛对DCM大鼠心功能的改善，其机制可能与增强T淋巴细胞免疫及降低炎症反应有关。

Objective To study astragalus polysaccharide＇s synergistic enhancement on Carvedilol＇s improvement effects on dilated cardiomyopathy＇s heart function. Methods SD rat model of dilated cardiomyopathy was established and divided into CON group （ no treatment）, CAR group （ only Carvedilol treat- ment） ,APS group （only astragalus polysaccharide treatment） and C ＋ A group （Carvedilol and astragalus polysaccharide treatment ） according to their treatment methods. The left ventricular ejection fraction （ LVEF）, Tei index, high sensitive C reactive protein （ hs-CRP）, tumor necrosis factor （TNF） -a, interleukin （IL）-1 ,IL-6, IL-8, CD3 ~ , CD4 ~ and CD8 ~ T lymphocyte subsets in four groups were detected and compared. Results The LVEF in C ＋ A group and CAR group were significantly higher than those in CON group and APS group respectively （ P 〈 0. 05, respectively）, while Tel index in C ＋ A group and CAR group were significantly lower than those in CON group and APS group respectively （P 〈0. 05 ,respectively）. The LVEF in C ＋ A group was significantly higher than that in CAR group （ P 〈 0. 05 ）, while Tel index in C ＋ A group was significantly lower than that in CAR group （P 〈0. 05）. The hs-CRP,TNF-ct,IL-1 ,IL-6 and IL-8 in C ＋ A group and APS group were significantly lower than those in CON group and CAR group respectively （P 〈 0.05, respectively）, while CD3 ＋ , CD4 ＋ and CD8 ＋ T lymphocyte subsets in C ＋ A group and APS group were significantly higher than those in CON group and CAR group respectively （ P 〈 0.05, respective- ly）. The hs-CRP,TNF-α,IL-1 ,IL-6 and IL-8 in C ＋ A group were significantly lower than those in APS group respectively （ P 〈 0. 05, respectively）, while CD3 ＋ , CD4 ＋ and CD8 ＋ T lymphocyte subsets in C ＋ A group were significantly higher than those in APS group respectively （ P 〈 0. 05, respectively）. Conclusion Astragalus polysaccharide has significant synergistic enhancement on Carvedilol＇s improvement effects on dilated cardiomyopathy＇s heart function, which may be related to the enhancement of T lymphocyte immunity and the decreasing of inflammatory reaction.