不同分子量壳聚糖对高脂膳食小鼠血糖的调节作用

The glucose regulation effect of different molecular weight chitosan on mice fed high-fat diet

为研究不同分子量壳聚糖对高脂膳食小鼠血糖的调节作用,选取C57BL/6J雄性小鼠48只,随机分为4组:正常组(Control)、高脂组(high fat,HF)、低分子量壳聚糖组(high fat+low weight molecular chitosan,HF+LWMC)和高分子量壳聚糖组(high fat+high weight molecular chitosan,HF+HWMC)。每周记录其摄食量和体重。饲养16周后,对小鼠血清、肝脏的生化指标进行测定,并对肝脏磷酸烯醇式丙酮酸(Phosphoenolpyruvate carboxybinase,PEPCK)及葡萄糖-6-磷酸酶(Glucose 6phosphatase,G6Pase)的基因表达进行检测。结果表明:壳聚糖的添加可降低高脂膳食小鼠的体重和血脂水平,缓解肝脏脂质过氧化,高分子量壳聚糖的效果略好于低分子量壳聚糖。

In order to compare the glucose regulation effect of different molecular weight chitosan on mice fed high-fat diet, 48 male C57BL/6J mice were randomly assigned to four groups, The control group （Control）, which consumed a normal diet, The HF group （HF）, which was fed with a high fat diel, The HF＋LWMC and HF ＋HWMC groups, which were fed with a high-fat diet supplement with 5 % low weight molecular chitosan and 5 % high weight moleeu lar chitosan, respectively. The diet intake and weight data were col leered weekly. The biochemical indicators of serum and liver and thegene expression of PEPCK and G6Pasc were determined at tbe end of the 16 week. The results indicated that supplementation of chitosan could reduce body weights, decrease the levels of serum lipid, and al leviate the liver lipid peroxidation of HF mice. Also, high weight mo- lecular ehitosan showed better hypolipidemic effect than low weight molecular chitosan.