南山茶饼中乙醇提取物5个多酚化合物的分离纯化

Isolation,purification and identification of extracts from seed of Camellia semiserrata Chi.

为综合开发利用油茶资源,对南山茶饼乙醇提取物的组分进行了制备和鉴定。将经过乙酸乙酯萃取和硅胶柱层析分离纯化收集的组分XVII、XXVII、XXVIII和XXXIV 4个组分进行了HPLC制备,得到了纯度分别为99.2%,95.4%,97.5%,99.1%,97.4%的5个化合物。用安捷伦高分辩率质谱工作站Qualitative Analysis B.06.00分子式计算器对质谱准分子离子碎片进行了化学式的计算,结合化合物的裂解规律和化合物物理化学性质,鉴定为原儿茶酸和4个黄酮类化合物。化合物的核磁共振鉴定将在另外的文章中报道。

To comprehensively develop and utilize Camellia resources, fractions of ethanol extract of Camellia semiserrata Chi, cake were separated and identified. Five purified compounds with pu- rity of 99.2%, 95.4%, 97.5%, 99.1% and 97.4%, respectively, were achieved. By using affiliated molecular formula of generator by agilent high resolution mass spectrometry workstation Qualitative Analysis B. 06.00, formula of MS fragmentation of molecular ions were calculated, combining with fragmentation patterns of compounds and physical and chemical properties, the compounds were identified as protocatechuic acid and four kinds of flavonoids.