杏鲍菇多肽提取工艺优化及其抗氧化活性研究

Optimization Extraction of polypeptide from Pleurotus eryngii mycelium and Its Antioxidation in vitro

为研究杏鲍菇菌丝体多肽提取工艺及其抗氧化活性,以杏鲍菇菌丝体多肽提取率为考查指标,在单因素试验的基础上采用Box-Behnken方法,通过响应面设计优化提取菌丝体多肽的工艺,用Sephadex-50葡聚糖凝胶对所提取的多肽进行纯化,并测定其还原力及清除DPPH·、O_2^-·、·OH能力。结果表明,在试验范围内各因素对杏鲍菇菌丝体多肽提取率的影响程度从大到小依次为:pH值>料液比>提取时间;杏鲍菇菌丝体多肽的最佳提取工艺参数为pH值8.85,提取时间4.17h,提取料液比111.86(g/mL),在该工艺条件下,多肽的提取率为50.88%;杏鲍菇菌丝体多肽具有一定的抗氧化能力,并与多肽质量浓度呈一定的量效关系。

The optimal extraction conditions of polypeptide from Pleurotus eryngii mycelium was ivestigated, and activity of the an tioxidant was evaluated in this study. Based on the single factor tests, the extraction conditions of polypeptide from P. eryngii myce- lium were optimized by Response Surface Methodology（RSM） to in- crease the extraction rate of polypeptide. Moreover, the polypeptide was purified with Sephadex-50, and the antioxidant activity of poly peptide was assessed using several assay model systems in vitro. The optimal extraction conditions of polypeptide were pH 8. 85, extraction time 4.17 h, liquid-to material ratio 1 ： 11.86 （g/mL）. Under the optimal extraction conditions, the maximum extraction rate of polypeptide was 50.88%. Extracted polypeptide showed good antioxidant activity in a dose-dependent manner.