摘要
采用超声波辅助提取,结合HPLC-ICP-MS分析手段,分别以温度、时间、提取剂用量为单因素研究其对萃取TBT的影响。以这3个因素做正交试验,建立贻贝中三丁基锡测定的新方法。最优条件为温度35℃,时间30 min,萃取剂用量30 m L。以贻贝,大黄鱼和虾为典型代表物,在最佳条件测得三丁基锡回收率分别为90.55%,86.15%和88.25%。三丁基锡在0~100μg/L的浓度范围内线性关系良好,其线性回归方程y=3 857.0x-5 528.2,线性相关系数R=0.9990,仪器的检测限为1μg/L。
The effect of ultrasonic extraction process in seafood sample by HPLC-ICP-MS was studied in this paper.Extraction conditions such as extraction temperature, extraction time and volume of extraction solvent were optimized by an orthogonal array experimental design. The optimal conditions for temperature 35 ℃, 30 min, 30 mL This proposed procedure was successfully applied to the determination of TBT in dried mussel, shrimps and yellow croaker with satisfactory results, the average recoveries were in the range of 90.55%, 86.15% and 85.25%. The TBT behaved linearly in the 0- 100 μg/L, concentration range with the coefficients is 0.9990. The limit of instrument detection for TBT is 1 μg/L.
作者
杨华
刘丽君
朱艳杰
戚向阳
张慧恩
潘道东
Yang Hua Liu Lijun Zhu Yanjie Qi Xiangyang Zhang Huien Pan Daodong(Ningbo University, Ningbo 315211, Zhejiang Zhejiang Wanli University, Ningbo 315100, Zhejiang)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第3期268-275,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
国家支撑计划(2015BAD17B02-3)
浙江省水产重中之重学科开放基金项目
