二甲亚砜对酵母多糖致肠道屏障损伤的保护作用

Protective effects of dimethyl sulfoxide on zymosan-insulted gut barrier impairment

目的:探讨二甲亚砜(DMSO)对酵母多糖诱导的肠道炎性因子释放及肠屏障功能损伤的影响。方法:SD大鼠随机分为4组:生理盐水组(SS组),DMSO组(DS组),酵母多糖+生理盐水组(ZS组)和酵母多糖+DMSO组(ZD组)。每组又分为伤后4 h和24 h两个亚组。检测肠组织细胞因子TNF-α和IL-10含量和血中二胺氧化酶(DAO)活性,并对肠损伤程度进行组织学评分。结果:与ZS组相应时间点相比,ZD组肠组织TNF-α含量减少、IL-10含量增加,血中DAO活性降低,肠绒毛损伤减轻、肠损伤评分降低。结论:DMSO可抑制酵母多糖引起的肠组织炎性因子释放,减轻肠屏障损伤程度。

Objective： To explore the effect of dimethyl sulfoxide（DMSO） on suppressing the release of gut inflammatory cytokine and re- store of the barrier impairment following zymosan-insulted systemic inflammatory response syndrome （SIRS）. Methods： S D rats were randomly divided into four groups： sham with administration of normal saline （SS group） ; sham with administration of DMSO （DS group） ; zymosan with administration of normal saline （ZS group） ; and zymosan with administration of DMSO （ZD group）, each group includes two subgroups at 4 h and 24 h after surgery. At 4 h and 24 h after intraperitoneal injection of zymosan （750 mg/kg）, the levels of intestinal inflammatory cytokines （tumor necrosis factor-or and interleukin-10） and the activity of diamine oxidase in plasma were examined. Intestinal injury was evaluated by using an intestinal histological score. Results： DMSO suppressed the release of tumor necrosis factor-a and increased interleukin-10 levels in the intestine compared with the ZS group at the corresponding time points. DMSO decreased the level of diamine oxidase in plasma compared with the ZS group. DMSO restored the injury of intestinal villi and the gut injury score was significantly lower than that in the ZS group. Conclusion： DMSO can suppress the release of intestinal inflammatory cytokines and restore zymosan-insulted gut barrier impairment.