片仔癀肝宝对慢性肝损伤凋亡的影响

Effect of Pianzaihuang Ganbao on the Apoptosis of Chronic Liver Injury

目的:探讨片仔癀肝宝(肝宝)对CCl4诱导大鼠慢性肝损伤凋亡的影响。方法:60只SD大鼠随机分为正常对照组、模型组、西利宾胺组、肝宝低剂量组、肝宝中剂量组、肝宝高剂量组,每组10只。除正常对照组外,SD大鼠腹腔注射50%CCl4橄榄油溶液1 ml/kg,2次/周,连续8周。于造模第四周开始给药,设正常对照组(生理盐水)、模型组(生理盐水)、阳性药对照组(西利宾胺,50 mg/kg),肝宝低剂量组(150 mg/kg)、肝宝中剂量组(300 mg/kg)、肝宝高剂量组(600 mg/kg),1次/d,连续4周。于末次给药24 h后,HE染色观察肝宝对肝组织病理学的影响,TUNEL法观察肝组织凋亡情况,比色法检测Caspase-3和Caspase-9的变化,western blot检测肝宝对Bax和Bcl-2蛋白表达的影响。结果:HE染色发现,各用药组均可不同程度缓解CCl4诱导的肝损伤;肝宝抑制肝细胞凋亡;降低Caspase-3和Caspase-9的活力;下调Bax表达,上调Bcl-2表达。结论:片仔癀肝宝可以抑制CCl4诱导的肝细胞凋亡。

Objective： To explore the effect ofPianzaihuang Ganbao （GB） on the apotosis of chronic liver injury induced by CC14 in rats. Methods： Sixty Sprague - Dawley （SD） rats were randomly divided into control, model, silymarin, GB-low, GB-middle and GB-high groups, ten rats per group. Except the control group was received olive oil, the rest groups were intraperitoneally injected with CCL 1 ml/kg, twice a week for 8 weeks constitutively. At the fourth week, the model and the control group were orally administration physiological saline （PS）, while the silymarin （50 mg/kg）, GB-Low （150 mg/kg）, GB-middle （300 mg/kg）, GB-high （600 mg/kg） were orally administrated in treatment group respectively for 4 weeks. Twenty-four hours after the last treatment, HE staining was used to observe the histopathological change in liver; the apoptosis was determined by TUNEL assay; Caspase-3 and Caspase-9 were detected; the protein expression of Bax and Bcl-2 were determined by western blot. Results： The results from HE staining found that all treatment could alleviate the injuried liver tissues differently; GB could inhibit the apotosis and decrease the activity of Caspase-3 and Caspase-9; furthermore, down-regulated the expression of Bax and up-regulated Bcl-2. Conclusion： GB could inhibit the apotosis induced by CC14.