成年辽宁绒山羊皮肤毛囊周期性发育不同时期FGF_5 mRNA的表达分析

Expression of FGF_5 mRNA in Hair Follicles of Adult Liaoning Cashmere Goats in Different Periods

成纤维细胞生长因子(FGF_5)基因在次级毛囊的周期性发育过程中具有重要的调控作用。利用RT-PCR技术检测了FGF_5基因mRNA在成年辽宁绒山羊皮肤毛囊不同时期(兴盛前期、兴盛期、退行期和休止期)的表达规律。结果表明:FGF_5基因mRNA在不同时期的辽宁绒山羊皮肤组织中均有表达,表达量由高到低顺序依次为休止期、兴盛期、退行期和兴盛前期;在休止期表达量最高,兴盛前期表达量最低;其中休止期表达量与兴盛前期和退行期间差异均显著(P<0.05),兴盛前期与其余3个时期间也差异显著(P<0.05),但休止期与兴盛期、兴盛期与退行期间差异均不显著(P>0.05)。因此,FGF_5基因表达可能控制着绒山羊的毛囊从兴盛期向退行期转换。

FGF5 plays an important role in the developmental processes of secondary follicle. Using RT-PCR technology, this study was conducted to detect the expression pattern of FGF5 mRNA of adult Liaoning cashmere goats in different periods. The result showed that FGF5 gene mRNA were expressed in skin tissue of Liaoning cashmere goats in different periods,while the expression levels, from high to low,were telogen, anagen, catagen and proanagen, respectively.The expression level reached a peak in telogen and reached minimum peak in proanagen. The expression level between telogen and proanagen,catagen had significant differences （P〈0.05）,andthedifferencebetweenproanagenandtheanagenwas significant （P〈0.05）.