外源性白细胞介素-10对变应性鼻炎大鼠STAT3蛋白活化的影响

Effects of exogenous interleukin-10 on STAT3 protein activation in rats with allergic rhinitis

目的探讨外源性白细胞介素-10（IL-10）对变应性鼻炎大鼠（AR）信号传导及转录激活因子3（STAT3）蛋白活化的影响。方法 SD大鼠60只,采用随机数字表法将其分为模型AR组、IL-10组和对照组,每组20只。AR组和IL-10组采用常规卵白蛋白（OVA）联合氢氧化铝致敏法建立AR大鼠模型,阴性对照组采用生理盐水代替,于最后一次激发后48 h,ELISA试剂盒检测血清中Ig E和鼻黏膜中IL-6、TGF-β和P-STAT3的含量变化,同时用Western-blot法检测鼻黏膜中IL-6、TGF-β和P-STAT3的表达变化。结果AR组大鼠均表现出四处摩擦、涕流满面等重度AR的临床表现,IL-10组表现明显减轻（P〈0.05）;与对照组相比,血清中Ig E和鼻黏膜中IL-6、TGF-β和P-STAT3的含量均明显升高（P〈0.05）,经IL-10干预后,各指标均明显降低（P〈0.05）;Western-blot显示,与对照组相比,AR组和IL-10组大鼠鼻部组织中IL-6、TGF-β和P-STAT3表达水平明显升高,而IL-10组大鼠鼻部组织中各指标的表达水平低于AR组。结论用外源性IL-10的方法治疗AR,可明显缓解AR的临床表现,抑制STAT3活化免疫应答,可在一定程度上达到治疗AR的目的。

Objective To investigate the effect of exogenous interleukin-10 （IL-10） on STAT3 protein activation in rats with allergic rhinitis （AR）.Methods60 SD rats were randomLy divided into AR model group, IL-10 group and control group, with 20 in each group.AR group and IL-10 group were treated with conventional ovalbumin （OVA） combined with aluminum hydroxide sensitization method.The negative control group was treated with saline instead of 48 h after the last challenge.The levels of IL-6, TGF-β and P-STAT3 in nasal mucosa and serum IgE were measured by ELISA kit.The expression of IL-6, TGF-β and P-STAT3 in nasal mucosa was detected by Western-blot Detection.ResultsAR group exhibited severe clinical manifestations of AR, such as friction around, tears flowing, the signs of which in IL-10 group were significantly lower than that in the control group （P〈0.05）.Compared with the control group, the levels of serum IgE and IL-6, TGF-β and P-STAT3 in the nasal mucosa were significantly higher than those in the control group （P〈0.05）.After treatment with IL-10, the levels of TGF-β and P-STAT3 were significantly increased （P〈0.05）.Western-blot showed that compared with the control group IL-6, TGF-β and P-STAT3 in nasal tissues of AR group and IL-10 group were significantly increased, while the expression level of IL-10 group was lower than that of AR group.ConclusionTreatment of AR with exogenous IL-10 can significantly relieve the clinical manifestation of AR, inhibit STAT3 activation immune response, and achieve the purpose of treatment of AR in some extent.