大强度运动导致肝脏线粒功能紊乱及姜黄素的拮抗效应

High-Intensity Excercise Induced Hepatic Mitochondrial Dysfunction in Mice and Protective Effects of Curcumin

目的:探讨姜黄素对大强度游泳运动小鼠肝脏线粒体功能紊乱的拮抗作用。方法:成年雄性BALB/C小鼠随机分为安静对照组、运动对照组、运动+姜黄素组[100 mg/(kg·d)]和安静+姜黄素组[100 mg/(kg·d)]。干预期为4w,干预期最后1w同时进行游泳运动训练,每天训练90 min,每天采用上述方式游泳运动7d,动物末次运动完成后处死。观察肝脏超微病理形态改变,测定血清谷丙转氨酶、谷草转氨酶水平,以及肝脏线粒体膜电位、呼吸控制率等线粒体功能学指标。结果:与安静对照组相比,姜黄素干预明显抑制了大强度运动导致的小鼠血清谷丙转氨酶(P<0.05)和谷草转氨酶(P<0.05)水平的上升,减轻了运动导致的肝细胞线粒体超微病理结构异常。姜黄素干预显著抑制了运动导致的小鼠肝脏线粒体膜电位(P<0.05)和呼吸控制率水平(P<0.05)的下降。结论:姜黄素对大强度运动小鼠肝细胞线粒体超微结构损伤和功能紊乱具有良好的拮抗作用,为天然植物化学物应用于抗运动疲劳提供新的前景方向。

Objective To investigate the protective role of curcumin in intense swimming-triggered dysfunction focused on hepatic mitochondria.Method Adult male BALB/C mice were randomly divided into four groups including rested controls,intense exercise,intense exercise＋curcumin（100 mg/kg body weight）and rested plus curcumin（100 mg/kg body weight）pretreatment.Curcumin was administrated for 4 weeks and intense exercise program（swimming for 90 min/d）was performed concurrently at the fourth week.Immediately upon final swimming,the mice were sacrificed.Serum alanine aminotransferase（ALT）,aspartate transaminase（AST）,and hepatic ultrastructural malformation were examined.Hepatic mitochondrial membrane potential（MMP）and respiratory control ratio（RCR）were measured.Result Compared to swimming group,curcumin intervention decreased intense swinmming-triggered the leakage of ALT（P〈0.05）and AST（P〈0.05）and hepatic ultrastructural malformation.Curcumin pretreatment evidently alleviated mitochondrial dysfunction by inhibiting the decline of membrane potential（P〈0.05）and respiratory control ratio（P〈0.05）in hepatic mitochondria of intense swimming mice.Conclusion Curcumin exhibited protective role in intense exercise-induced hepatic mitochondrial ultrastructural damage and dysfunction,highlighting a promising strategy for sports anti-fatigue by naturally occurring phytochemicals.