黄芩苷对黏菌素致小鼠周围神经毒性的保护作用研究

Baicalin Protects Against Colistin-induced Peripheral Neuro Toxicity in Mice

黏菌素的神经毒性是众所周知的。中药黄芩苷具有抗氧化、保护神经等作用。本试验通过设定正常组(生理盐水)、模型组(静脉注射15 mg/kg体重黏菌素)、黄芩苷高剂量组(200 mg/kg体重黄芩苷+15 mg/kg黏菌素)、黄芩苷中剂量组(100 mg/kg体重黄芩苷+15 mg/kg体重黏菌素)和黄芩苷低剂量组(50 mg/kg体重黄芩苷+15 mg/kg体重黏菌素)共5个剂量组,连续给药7 d后,进行氧化-抗氧化相关生化指标测定(MDA、SOD、CAT和GSH)、坐骨神经组织中凋亡相关因子表达(Caspase3和Bax)及碱性髓鞘蛋白(myelin basic protein,Mbp)表达的测定。结果表明,黄芩苷干预组的小鼠氧化指标明显降低,提高小鼠的抗氧化能力;Caspase3和Bax以及Mbp蛋白表达量均有降低。

Colistin-induced neurotoxicity is well - known. Baicalin has a lot of pharmacological effects, such as antioxidation, neuro-protection, et al. In this Study, mice were divided into five dose groups： normal group （saline）, model group （intravenous colistin 15 mg/kg）, baiealin high close group （200 mg/kg baicalin ＋ 15 mg/kg colistin）, and baicalin media dose group （ 100 mg/kg baicalin ＋ 15 mg/kg colistin）, baicalin low dose group （50 mg/kg baicalin ＋ 15 mg/kg colistin）. After 7 days of administration, we measured the oxidation-antioxidant, expression of apoptosis-related factors （caspase3 and Bax） and myelin basic protein detection in sciatic nerve. The results showed that： baicalin significantly reduced reactivity of oxygen indicators and improved the anti-oxidative capacity The expression of Caspase3, Bax and Mbp proteins were significantly decreased by baicalin.