山西地区猪流行性腹泻免疫情况调研

Investigation of immune state of porcine epidemic diarrhea in Shanxi

猪流行性腹泻近年来严重危害养猪业发展,为了查明不同免疫程序对防控本病的效果,对山西各地市10个规模化种猪场母猪疫苗免疫情况、免疫程序及防控效果等进行了调研。结果显示,未免疫疫苗的猪群乳猪死亡率为61.70%,而母猪产前40 d和20 d后海穴注射胃流轮三联活苗,肌肉注射胃流二联灭活苗的猪群乳猪死亡率为10.26%;母猪产前40 d和20 d后海穴注射胃流轮三联活苗,3日龄乳猪后海穴注射胃流轮三联活苗的猪群乳猪死亡率为9.63%;应用PCR检测病原,PEDV、TGEV的阳性率分别为64.1%和2.56%;ELISA检测母猪乳汁中IgA抗体,阳性率为95%;检测母猪、乳猪、断奶仔猪血样中IgA抗体,阳性率为30%。通过本次调研与检测,筛选出两种较好的免疫程序,为猪场防控该类疫病提供参考。

In recent years, the porcine epidemic diarrhea （PED） has caused huge economic losses to the swine industry. In order to ascertain the prevention-control effect of different immune procedures against PED Virus（ PEDV）, our research group investigated the vaccine type, immune procedure and prevention-control effect of 10 scale pig breeding farms in different cities Shanxi province . The results showed that the mortality rate of sucking piglets in non-vaccine immunity groups was 61.70%. However, and it was 9.63 % when the pregnant sows immunized with three-combined live vaccine of the transmissible gastroenteritis virus （TGEV）, PEDV and porcine rotavirus via houhai acupoint at 40d and 20d before farrowing, respectively, and the same immune method was used for 3d-sucking peglets. We detected pathogens by PCR, and the positive rate of PEDV and TGEV was 64.1% and 2.56%, respectively. The positive rates of IgA antibody against PEDV from colostrum of sows and sera of sows ,sucking piglets and postweaning pigs were 95% and 30% detected by Elisa. Based on the statistical analysis of these results, we selected out the two best immune procedures to provide references for the prevention and control against PEDV in pig farms.